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Luminex Blog
Precision Coupling with Molar Amounts of Protein
Precision coupling is useful for multiplex antibody assays, screening recombinant antibodies, and more!
When coupling whole immunoglobulins or antibody fragments for sandwich capture assays, coupling μg amounts of protein per million beads is the best way to optimize the amount of antibody needed to develop the most sensitive assay. However, to develop a multiplex assay for antibody titer or antigen specificity determination, it is best to couple antigens with the same moles per million beads. This is especially true when unrelated antigens of different molecular weights show some degree of cross-reactivity.
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xMAP TECHNOLOGY OVERVIEW
xMAP Technology makes up the basis of Luminex’s core business. This flexible technology is centered on the use of Luminex xMAP Microspheres.