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xMAP® Antibody Coupling Kit

Antibody Coupling Kit

Everything you need to couple your capture antibody of interest to MagPlex® microspheres

xMAP® Antibody Coupling Kit

 

The xMAP® Antibody Coupling Kit, or “AbC” kit, is a reagent kit that contains everything a researcher needs to couple an antibody to MagPlex® Microspheres. Similar to an ELISA plate, MagPlex Microspheres can be used as the substrate on which immunoassays can be performed. Unlike a traditional ELISA, however, this assay method requires much less antibody, sample, and time; with equal or better sensitivity and dynamic range.1

 

xMAP Antibody Coupling KitSince each microsphere is uniquely addressable, multiple immunoassays can be performed simultaneously with the same sample, in the same well. Once your desired antibody is coupled to the surface of the bead you can begin optimizing monoplex or multiplex immunoassays to suit your needs.

 

Experience the benefits of immunoassays on xMAP® Technology, including:

  • Less sample
  • Less antibody (up to 90% less antibody in some cases2)
  • Less labor
  • Greater reproducibility
  • Equal or better sensitivity and dynamic range

 

Benefits

 

  • Conveniently includes all reagents and consumables necessary to couple antibodies to MagPlex microspheres
  • Easy to use protocol

 

Recommended Complementary Products

 

 

Specifications

 

Kit Contents

  • Easy to follow coupling protocol, including guidance on how to assess coupling efficiency
  • Pre-optimized reagents and buffers, including EDC3
  • All required consumables (i.e. reaction tubes and transfer pipettes)

 

Run Times

  • Hands-on time = ~40mins
  • Total turn-around time = ~3hrs

 

Kit Capacity

  • Small scale: up to 10 reactions of 5×106 beads/reaction or less
  • Large scale: up to 4 reactions of greater then 5×106 beads/reaction
  • Maximum scale is 12.5×106 beads/reaction

 

Number of Beads that can be Coupled per Kit

  • Maximum: 50×106 beads (4 reactions @ 12.5×106 beads/reaction)
  • Minimum: 2.5×106 beads (1 reaction @ 2.5×106 beads/reaction)

Workflow

 

xMAP Antibody Coupling Kit Workflow Overview for Use with MagPlex Microspheres:

 

xMAP Antibody Coupling Kit Workflow Overview for use with MagPlex Microspheres

Ordering Information

 

Order Now
If you have any questions or require further assistance placing your order, please contact our Order Management Team.
Email: [email protected]
Phone: 1-512-336-3550
Toll Free: 1-866-401-5450
Fax: 1-512-219-0544
Fax (Europe, Middle East, Asia, India): +31 (0)73 800 1998
Reagent Shipping Policy
xMAP Antibody Coupling Kit orders ship within three business days of order receipt. However, backordered items may have longer than normal lead times. This kit has a shelf life of one year from the date of manufacture. All xMAP AbC kits are guaranteed to have a minimum remaining shelf life of one month from the date they ship.
Part Number
40-50016

This kit is limited to antibody coupling and is NOT intended to be a comprehensive assay development kit.

This kit is specifically designed for coupling antibodies to MagPlex microspheres. Please visit the Resources section of the website to learn about coupling nucleic acids, antibodies, and other proteins to both MicroPlex® and MagPlex microspheres.

 

  1. xMAP® vs ELISA whitepaper
  2. For more information on how xMAP stacks up against ELISA, see the xMAP vs. ELISA White Paper.
  3. Kit does NOT include microspheres or antibodies.