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xMAP® Molecular STEC Serotyping Assay

xMAP® Molecular STEC Serotyping Assay

Qualitative multiplex PCR assay for simultaneous determination of STEC serogroups

The Luminex xMAP® Molecular Shiga Toxin-producing Escherichia coli (STEC) Serotyping Assay is a qualitative multiplex PCR assay for simultaneous determination of STEC serogroups and important virulence markers. It is a multiplex, nucleic acid-based assay for research and epidemiological use in identifying the serogroup of a STEC isolate.

 

Features include:

 

  • Flexible Throughput: A few samples to a full 96-well plate
  • Easy Workflow: Compatible with MAGPIX® or Luminex® 200™ instruments
  • Saves Time: Less time to results compared to traditional antisera-based testing and single-plex testing of multiple samples
  • Increased Targets: Now also detects two important virulence markers
  • Expandable: Able to run in conjunction with Shiga toxin gene testing or direct Shiga toxin testing
  • Preferred Laboratory Procedure: Noted in the FDA Bacterial Analytical Manual (BAM)1

 

Kit Targets

 

Serogroup/Marker Comments
O157 Most common in U.S.
O26, O45, 0103, O111, O121, O145 “Big 6″—the cause of more than 70% of U.S. infections
O91, O113, O128 May be associated with severe disease
O104 Source of largest hemolytic uremic syndrome (HUS) outbreak worldwide2
eae Intimin gene—important for cell attachment
aggR Transcriptional regulator for other factors

xMAP® Molecular STEC Serotyping Assay Workflow

xMAP Molecular STEC Serotyping Assay - Workflow Step 1

Step 1

Sample Preparation

~15 minutes

xMAP Molecular STEC Serotyping Assay - Workflow Step 2

Step 2

PCR

~100 minutes

xMAP Molecular STEC Serotyping Assay - Workflow Step 3

Step 3

Hybridization

~30 minutes

xMAP Molecular STEC Serotyping Assay - Workflow Step 4

Step 4

Add Reporter / Incubation

~15 minutes

xMAP Molecular STEC Serotyping Assay - Workflow Step 5

Step 5

Detection

<1 minute/sample

Ordering Information

 

Luminex Online Order Management
Email: [email protected]
Phone: 1-512-336-3550
Toll Free: 1-866-401-5450
Fax: 1-512-219-0544
Fax (Europe, Middle East, India, Asia): +31 (0)73 800 1998
Product Name Kit Size Registration Status Part Number
xMAP Molecular STEC Serotyping Assay

Includes Primers, Bead Mix, SAPE, TMAC 1.5X, and TMAC 1X

100 Tests Research Use Only (RUO) 40-00031
  1. BAM: Diarrheagenic Escherichia coli. FDA (Internet). Cited 2017 Oct. Available from: https://www.fda.gov/food/foodscienceresearch/laboratorymethods/ucm070080.htm.
  2. EHEC O104:H4 in Germany 2011: Large Outbreak of Bloody Diarrhea and Haemolytic Uraemic Syndrome by Shinga Toxin-Producing E. coli via Contaminated Food.” Improving Food Safety Through a One Health Approach: Workshop Summary., U.S. National Library of Medicine. Cited 2017 Nov. Available from: https://www.ncbi.nlm.nih.gov/books/NBK114499/.

 

For Research Use Only. Not for use in diagnostic procedures. This assay method has not been independently evaluated by a third party certifier of government agency. Please contact [email protected] to obtain the appropriate product information for your country of residence.