Muse® STAT1 Activation Dual Detection Kit

Optimized for ease of use and deeper, faster scientific insights

 

The Muse® STAT1 Activation Dual Detection Kit – 100 Tests (Part Number: MCH200113) is part of a series of products that include a pair of antibodies which bind to the same target; one antibody detects total protein expression and the other detects the phosphorylated form of the same target. Two-parameter analysis allows specific detection of phosphorylation, eliminating false positives while enhancing the signal-to-noise ratio.

 

The Muse STAT1 Activation Dual Detection Kit includes two directly conjugated antibodies—a phospho-specific anti-phospho-STAT1 (Tyr701)-PerCP and an anti-STAT1-AlexaFluor® 555 conjugated antibody—to measure total levels of STAT1. This two-color kit is designed to measure the extent of STAT1 phosphorylation relative to the total STAT1 expression in any given cell population. As a result, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of STAT1 activation after stimulation. Simultaneous measurement of both total and phospho-STAT1 confirms target specificity of the phosphorylation event.

 

The utilization of a total and phospho antibody duo performed in multiplex provides an enhanced and more reliable detection of the phospho STAT1 compared to the total STAT1 within a mixed population. The antibody pair provided in the kit has been carefully titrated to ensure its ability to measure total and phospho-STAT1 simultaneously on the same protein for accurate determination of protein level and activation. This kit contains optimized fixation, permeabilization, and assay buffers to provide researchers with a complete solution for STAT1 signaling analysis. Data generated using the Guava® Muse® Cell Analyzer with the Muse Software provides:

 

  • Percentage of inactivated cells
  • Percentage of activated cells (via phosphorylation)
  • Percentage of non-expressing cells

 

The Muse STAT1 Activation Dual Detection Kit is optimized for use with the Muse Cell Analyzer.

 

Instrument Compatibility:

 

 

For Research Use Only. Not for use in diagnostic procedures.

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Product

 

Muse® STAT1 Activation Dual Detection Kit – 100 Tests

 

Part Number

 

MCH200113

 

Ordering Information

 

Ordering information can be found here

 

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For Research Use Only. Not for use in diagnostic procedures.