Muse® EGFR/MAPK Activation Dual Detection Kit

Optimized for ease of use and deeper, faster scientific insights

 

The Muse® EGFR/MAPK Activation Dual Detection Kit – 200 Tests (Part Number: MCH200114) is designed to provide researchers with a quick and easy way to detect the activation of both the EGFR and MAPK signaling pathways simultaneously using the Guava® Muse® Cell Analyzer. The kit was designed and optimized using a well-known EGFR stimulant, EGF, on A431 cells as a model system. The kit can also be used with other human cell lines to investigate signaling and activation pathways under certain conditions. Data generated using the Muse Cell Analyzer along with the corresponding Muse Software module measures:

 

  • Percentage of negative cells (e.g., no activated pathways)
  • Percentage of EGFR pathway-activated cells
  • Percentage of MAPK pathway-activated cells
  • Percentage of Cells activated by both pathways (dual activation of both EGFR and MAPK)

 

The Muse EGFR/MAPK Activation Dual Detection Kit includes two directly conjugated antibodies—a phospho-specific EGFR (Tyr1173)-Alexa Fluor® 555 and a phospho-specific ERK1/2 (Thr202/204, Thr185/Tyr187)-PECy5 conjugated antibody—to simultaneously measure the activation of both key cell signaling pathways in testing samples by flow analysis. Together, two-color analysis of EGFR and ERK1/2 activation in multiplex provides a more reliable detection method for assessing the EGFR signaling pathway, and measuring the consequence of its activation by monitoring the MAPK signaling activity downstream from this event. This antibody pair provides a sensitive and valuable tool to simultaneously detect the phosphorylation of EGFR and ERK, allowing researchers to profile EGF-mediated MAPK activation within a cell type of interest. Alterations in these signaling profiles can be used to monitor the effect of a particular upstream treatment targeting the pathway.

 

The Muse EGFR/MAPK Activation Dual Detection Kit is optimized on the Muse System, which makes sophisticated, fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse Cell Analyzer, intuitive software provides detailed or summary analysis of your cell sample in a few short steps. Both antibodies provided in the kit are carefully titrated and optimized together to ensure maximal performance when run in multiplex, alleviating the need for any additional optimization. This kit contains optimized fixation, permeabilization, and assay buffers to provide researchers with a complete solution for EGFR/MAPK dual pathway activation signaling analysis.

 

Instrument Compatibility:

 

 

For Research Use Only. Not for use in diagnostic procedures.

 

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Product

 

Muse® EGFR/MAPK Activation Dual Detection Kit – 200 Tests

 

Part Number

 

MCH200114

 

Ordering Information

 

Ordering information can be found here

 

The following details will need to be included with your purchase orders in order to process them:

  • Company name
  • Name, phone number, and email address of purchasing agent
  • Name and phone number of the end user
  • Valid purchase order number
  • Quote reference number, where applicable
  • Complete billing and shipping addresses
  • Product number, description, pricing, and quantity
  • Net 30 payment terms or other agreed upon payment terms
  • FOB shipping point or other agreed upon shipping terms

 

Questions on Orders: [email protected].

 

Questions on Billing and Payments: [email protected].

 

For Research Use Only. Not for use in diagnostic procedures.