Muse® Cell Dispersal Reagent

Optimized for ease of use and deeper, faster scientific insights

The Muse® Cell Dispersal Reagent – 100 Tests (Part Number: MCH100107) is an enzymatic reagent that has been formulated to gently disaggregate the cells that have aggregated in clumps in order to obtain uniform suspensions for cell counting. Cell samples containing cell aggregates may not yield accurate or consistent results with the Muse Count & Viability Reagent alone due to differential staining in clumps. Additionally, cell aggregates may clog or result in coincident events, hampering the assay.1-3 Cell lines such as the Chinese Hamster Ovary (CHO-K1) cell line that have been adapted to suspension culture tend to form cell aggregates.4,5 The Muse Cell Dispersal Reagent is useful for disaggregating clumpy suspension cultures in combination with the Muse Count & Viability Reagents, and yields improved accuracy and consistency for count and viability data with the Muse Count & Viability Assay.2 Data generated with the Muse Software module provides:

  • Viable cell count (cells/mL)
  • Total cell count (cells/mL)
  • Percentage viability of sample

The Muse Cell Dispersal Reagent is optimized for use with the Guava® Muse® Cell Analyzer, which makes sophisticated, fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse Cell Analyzer, intuitive software provides detailed or summary analysis of your cell sample in a few short steps.

Instrument Compatibility:

For Research Use Only. Not for use in diagnostic procedures.

1. Muse®Count & Viability User’s Guide, Document Part No. 4600-3373. Luminex, 2019.

2. Magallanes E, Helsel C, Liu B, Yokobata K. Validating the Guava®ViacountCDRAssay. Technical Note, Guava Technologies Inc, 2003.

3. Phi-Wilson J, Harvey J, Goix P, O’Neill R. A technology for the rapid acquisition of cell number and viability. Am Biotechnol Lab. May 2001:34-36.

4. Shen BP, Bodnar CA, Manuel J, Siegel AC, Levy GA. Comparison of Serum-Free Suspension Culture Media for the Growth and Production of a Protein-Producing CHO Suspension Line. Application Note. Industrial BioDevelopment Laboratory.

5. Hernandez C, Christie A, MacNorton S, Wilson S, et al. Optimization of Cell Counting Throughput for the SAFC Raw Materials Characterization Program. SAFC, Lenexa.

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Product

Muse® Cell Dispersal Reagent – 100 Tests

Part Number

MCH100107

Ordering Information

Ordering information can be found here

The following details will need to be included with your purchase orders in order to process them:

  • Company name
  • Name, phone number, and email address of purchasing agent
  • Name and phone number of the end user
  • Valid purchase order number
  • Quote reference number, where applicable
  • Complete billing and shipping addresses
  • Product number, description, pricing, and quantity
  • Net 30 payment terms or other agreed upon payment terms
  • FOB shipping point or other agreed upon shipping terms

Questions on Orders: [email protected].

Questions on Billing and Payments: [email protected].

For Research Use Only. Not for use in diagnostic procedures.