Guava® Muse®

Guava® Muse®

An easy to use, simple, compact, robust instrument for your cell analysis needs

Sophisticated cell analysis doesn’t have to be complicated or costly. With the Guava® Muse® Cell Analyzer, you can now achieve highly quantitative results at a fraction of the price, effort, and time. The Muse Cell Analyzer packs 3-parameter analysis into a compact, easy to use benchtop device, making flow cytometry accessible to anyone, anytime. A user-friendly touchscreen interface, intuitive cell analysis software, and optimized assays work to simplify your research.

 

Features

 

  • Quantitative data at the single-cell level
  • Simple and effortless operation
  • Intuitive cell analysis software and touchscreen user interface
  • Rapid setup and analysis
  • Optimized Muse assays
  • Compact size: footprint of only 8 in x 10 in (20 cm x 25 cm)
  • Flow cytometry technology in an affordable instrument

 

The Muse Instrument has an integrated touchscreen and software for data acquisition and analysis using optimized Muse Assays. Its microcapillary flow cell is engineered for acquisition of both suspension and adherent cells 2-60 microns in diameter.

 

Muse uses fluorescent reagents and detection to measure three parameters for every cell, with little or no sample preparation required. Muse assays are available for precision cell counts, as well as single-cell measurement of critical cell parameters.

 

Click here to learn more about our kits and reagents.

 

Download a copy of the brochure here.

 

 

How the Muse Cell Analyzer Works

 

Muse Cell Analysis Principle

 

The Muse Cell Analyzer uses miniaturized fluorescence detection and microcapillary cytometry to deliver single-cell analysis, which is highly quantitative — especially when compared with traditional bulk methods like microscopy and Western blot.

 

Sample collection, acquisition, and analysis of results is achieved in just a few steps. The user interface is designed to be intuitive, so you spend less time with experimental setup and analysis for the critical cell health assays you need most.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Laser-based Fluorescence Detection

 

The system delivers high-performance cell analysis using a microcapillary technology and miniaturized optics which occupy one-tenth the space of a typical cytometer. Laser-based fluorescence detection of each cell event can evaluate up to 3 cellular parameters. As a result, Muse provides more quantitative results than non-flow based imaging systems, which only examine up to two parameters, are time-consuming, and provide less quantitative data.

 

Novel, Miniaturized Flow Technology

 

The Muse Cell Analyzer uses miniaturized fluorescent detection and microcapillary technology to deliver truly accurate, precise, quantitative cell analysis compared to other methods. Laser-based fluorescence detection of each cell event can evaluate up to 3 cellular parameters – cell size (forward scatter) and 2 colors (detected in the red and/or yellow channels).

 

The system uses a microcapillary and miniaturized optics, which occupy one-tenth the space of a typical cytometer. This means that the instrument occupies only an 8 in x 10 in (20 cm x 25 cm) footprint. A green diode laser is used for excitation, and a uniquely designed series of retro-reflective lenses provide maximum light capture and sensitivity.

 

Highly Intuitive Touchscreen Interface

 

Muse features a highly intuitive touchscreen interface that allows simple step-by-step operation, so easy that no flow expertise is required to run assays. The touchscreen prompts you through simple on-screen instructions and guides you though sample loading to simple setting adjustments to results—in just a few steps.

 

Software

 

Muse Software is designed to be simple enough for any user to follow setup instructions to achieve sample acquisition, on the first use. Assay-specific software modules include sample graphs to make population gating setup foolproof. Results are displayed in both graphical and statistical formats specific to each application, making analysis unambiguous.

 

Muse Cell Analyzer Reported Statistics on Viable Cells

 

The figure below shows the data output for the Muse Count & Viability Assay. Healthy Jurkat cells were mixed with heat-killed Jurkat cells and stained with Muse Count & Viability Reagent, and then analyzed on the Muse Cell Analyzer. Data output include summary data (not shown) and optional dot plots (shown here). Reported statistics include viable cells/mL, % viability, and the total cells/mL. The left hand dotplot shows viability vs. cell size; the right hand plot shows viability vs. nucleated cells.

 

 

 

 

Muse Cell Analyzer Performance

 

Cell quantitation and analysis techniques, such as hemocytometry, that are based on image analysis have limited sensitivity and objectivity, and sample sizes may be too small for statistical analysis. Muse assays use relative fluorescence and flow cytometry to assess thousands of cells per sample, rendering data that is more accurate, more precise, and more reliable than traditional methods.

 

Accurate Cell Concentrations

The Muse Cell Analyzer counts cells more accurately than manual hemocytometry or image-based automated analysis.

 

Multiple adherent and suspension cell types were harvested, diluted, and counted. Cell counts from all three methods were averaged to obtain “theoretical cell concentration”. Each point represents the average cell concentration of 3 replicates, and each data series was fit with linear regression. Muse cell analysis data was correlated with the theoretical concentration with slope closest to 1, indicating superior accuracy.

 

Precise Results

 

The Muse Cell Analyzer counts cells and measures viability more precisely with smaller coefficients of variation (%CV) than manual hemocytometry or image-based automated analysis. When compared with image-based automated counting methods and manual hemocytometers, the Muse Cell Analyzer exhibited a narrower range of %CVs and consistently provided variation of less than 10% over the entire range of samples tested. Higher %CVs were observed for the Trypan blue-based methods, particularly at lower cell concentrations.

 

 

Analysis Cell Concentration Viability
Average % CV % CV Range Average % CV % CV Range
Image-based Automated Counter 9.2% 1.2-23.3% 3.7% 0.8-12.1%
Manual Hemocytometer 9.2% 6.3% 4.5% 0.5-9.2%
Muse 4.0% 0.3-8.8% 2.2% 0.4-5.6%

 

The Muse Cell Analyzer provides superior precision for cell concentration and viability measurements, compared to Trypan blue-based analyses. Data are based on triplicate measurements of 30 cellular samples from suspension and adherent cell lines at multiple concentrations and viabilities.

 

Linear Results Across Multiple Cell Types

 

The Muse Cell Analyzer performs with exceptional accuracy across multiple cell lines and a wide sample concentration range.

 

 

The concentration measurements for serial dilutions of five representative cell lines are shown, and include both adherent and suspension cells. Each point represents the average of three replicates. Expected cell concentrations were calculated by measuring the stock sample concentration with the Muse Cell Analyzer, then dividing by the dilution factor to obtain theoretical concentrations of diluted samples. Data show the comparison of observed vs. expected cell concentrations.

Assays for Muse Cell Analyzer

 

For the assays you rely on most, we’ve developed optimized kits, validated for robust performance on the Muse Cell Analyzer. Typical cell preparation protocols have been condensed and simplified, so sample preparation is fast and easy. You don’t need to optimize any software settings – the Muse Instrument calculates all gating parameters and thresholds for you. Spend less time with experimental setup, avoid reagent waste, and save money.

 

Click here to view a list of available kits.

 

 

What method do you currently use for fundamental cell counting, cell health, and signaling assays?

 

 

Other Cell Analysis Techniques Muse Assays
Hemacytometer

  • Limited due to low-resolution dye uptake for viability determination
  • Subject to manual counting and calculation errors
Muse Count and Viability Assay

  • Precise, fluorescence-based live/dead cell discrimination
  • Microcapillary fluidics for direct cell counts with industry-leading accuracy
Brightfield Microscopy-based Automated Hemocytometer/Cell Analyzer

  • Low accuracy based on small number of cells analyzed, and limited to count and viability measurement
Muse Assays

  • Fluorescent reagents generate signal based on specificity for unique targets
  • Statistical significance achievable from rapid acquisition of thousands of cells
Western Blot and Other Bulk Assays

  • Population heterogeneity is lost when cells are pooled
  • Methods are time-consuming and require expertise
  • Reagents must be validated and optimized
  • Data is qualitative or semi-quantitative at best
Muse Assays

  • Single-cell data is obtained from every sample
  • Extremely simple ‘pipette only’ assays
  • Validated and optimized for multiple cell types
Large, Open-system Flow Cytometer

  • Reagents must be validated for application
  • Optimization is time-consuming and uses precious sample
  • Software settings must be determined independently
Muse Assays

  • Fluorescent reagents are chosen for compatibility with lasers and detectors and optimized on multiple cell types
  • Assays have validated, optimized sample preparation protocols and pre-set software settings

Customer Testimonials

 

As scientists, peer review is the gold standard by which we assess technologies, second only to trying out tools and reagents ourselves. Routine but essential cell analysis assays are faster, easier, and more data-rich with the Muse Instrument. Get more insight on the Muse Cell Analyzer from our customers.

 

Read the Muse Cell Analyzer Reviews

 

“My lab has been using the Muse for about one year now and I can say that the Muse is exceptional. The Muse cuts the time of manually counting by half… I would recommend the Muse to anyone who does manual counting.”

– T. Weaver, Duke University, USA

 

“I just started to use Muse Cell Analyzer, and I’m happy with it at least for now. The machine is user-friendly, easy to learn and maintain. The thing I have to mention is I had a good experience with the custom service. Larry is very helpful.”

– M. Zhao, University at Buffalo, USA

 

“We bought the Muse in order to do quick experiments without having the full set up of a FACS…We are able to do quick and accurate cell counts, then run assays all in the convenience of the lab. The results seem to be consistent with what we would be getting using the FACS.“

– M. de la Vega, University of Hawaii, USA

Resources

 

Expanded Analysis Capability of Guava Muse Cell Analyzer with New Open ModulesPoster


Performance of a High Sensitivity Multiplexed Assay for Malarial Antigen Detection on Multiple Cytometry PlatformsPoster

Flow Cytometry and Imaging

Luminex flow cytometers and cellular analysis instruments give you instant access to all facets of cellular phenotypes and morphology.

Amnis Cellstream
Amnis FlowSight
Amnis ImageStreamX Mk II
Guava easyCyte

 

Support

In addition to service support, our worldwide technical support team is available by email and telephone.

 
Guava Muse Support Page

 

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