Muse® Autophagy LC3-Antibody Based Kit

Optimized for ease of use and deeper, faster scientific insights

 

The Muse® Autophagy LC3-Antibody Based Kit – 200 Tests (Part Number: MCH200109) provides a quantitative solution for studying autophagy. This kit contains two key detection reagents to facilitate the monitoring of lipidated LC3-II in a given cell system, and works by:

 

  • Employing a selective permeabilization solution to discriminate cytosolic LC3 from autophagic LC3 by extracting the soluble cytosolic proteins while protecting LC3, which has been sequestered into the autophagosome
  • Utilizing an autophagy detection reagent (Autophagy Reagent A) to prevent the lysosomal degradation of LC3, allowing for its quantification by flow cytometry

 

The Muse Autophagy LC3-Antibody Based Kit includes an anti-LC3 mouse monoclonal antibody conjugated to Alexa Fluor® 555 to measure and track the levels of LC3 within the cell. The anti-LC3 Alexa Fluor® 555 conjugated antibody and autophagy enabling reagents have been carefully evaluated to ensure optimal performance, alleviating the need for any additional validation of the kit reagents. In addition to reagents A and B, this kit includes an assay buffer to provide researchers a complete solution for autophagy analysis. The autophagy detection reagents and antibody have been optimized together to deliver a reliable way to measure and discriminate between cytosolic and lipidated LC3, providing accurate measurements of the autophagic process. Data generated using the Guava® Muse® Cell Analyzer along with the corresponding Muse Software module provides statistical values, measuring:

 

  • Mean autophagy value (for both control and test samples)
  • Autophagy induction ratio (test sample fluorescence relative to control)
  • Percentage of cells with increased autophagy (test sample versus control)

 

The Muse Autophagy LC3-Antibody Based Kit is optimized for use with the Muse System, which makes sophisticated, fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse Cell Analyzer, the intuitive software provides detailed or summary analysis of your cell sample in a few short steps.

 

Instrument Compatibility:

 

 

For Research Use Only. Not for use in diagnostic procedures.

 

Documentation is coming soon. Need Service or Support? Contact Support

 

Product

 

Muse® Autophagy LC3-Antibody Based Kit – 200 Tests

 

Part Number

 

MCH200109

 

Ordering Information

 

Ordering information can be found here

 

The following details will need to be included with your purchase orders in order to process them:

  • Company name
  • Name, phone number, and email address of purchasing agent
  • Name and phone number of the end user
  • Valid purchase order number
  • Quote reference number, where applicable
  • Complete billing and shipping addresses
  • Product number, description, pricing, and quantity
  • Net 30 payment terms or other agreed upon payment terms
  • FOB shipping point or other agreed upon shipping terms

 

Questions on Orders: [email protected].

 

Questions on Billing and Payments: [email protected].

 

For Research Use Only. Not for use in diagnostic procedures.