What’s New with Luminex® xMAP® Technology Partners—October 2014
The world of xMAP® Technology is evolving and progressing–our partners are releasing new products for use with your xMAP instruments. We want to keep you updated on the latest information provided by some of our partners.
Life Science Research Partners:
New Ab-conjugated Bead targets for use with Luminex® Histone H3 PTM Multiplex Assays:
Active Motif’s continuing expansion of modified histone targets for analysis using the Histone H3 PTM Multiplex Assay, the first epigenetic assay with multiplexing capability, now includes antibody-conjugated bead sets for H3K56ac, H3K27ac and H3T11ph histone modifications. The Histone H3 PTM Multiplex Assay enables high-throughput, high content screening of changes in histone modification levels using smaller sample amounts, and in less time and at a lower cost than traditional Western blot or immunofluorescence. The multiplexing capability is highly advantageous for screening assays because it provides the ability to interrogate both specific and off-target effects simultaneously.
Modification-specific antibody-conjugated beads are packaged individually to allow researchers to customize their assays for singleplex or multiplex analysis simply by selecting the antibody-conjugated beads for their post-translational modification (PTM) of interest. Each antibody-conjugated bead set used to capture modified histones has its own unique fluorescent label, enabling antibodies for multiple histone PTMs to be added to the same sample for multiplexing. A biotinylated reporter antibody is used to detect and measure the level of histones bound to the bead and produce a fluorescent signal following the addition streptavidin-phycoerythrin.
As the beads pass through the internal fluidics of the Luminex instrument, the fluorescent signals emitted by each bead are read to determine the bead identity and provide a readout of the streptavidin-phycoerythrin signal that is proportional to the amount of histone PTM captured by each bead set. The inclusion of Histone H3 Total Antibody-conjugated Beads into the multiplex reaction enables normalization against total H3 levels for determining relative histone PTM levels across samples.
QuantiGene® Plex Assay Pathway Panels
QuantiGene® Plex Assays use Luminex® xMAP® technology to simultaneously measure up to 80 genes in one well. Unlike qPCR, QuantiGene® Plex (QGP) utilizes branched DNA signal amplification, so RNA purification, cDNA synthesis, and PCR amplification are NOT required. Affymetrix now offers predesigned QGP pathway panels in the following areas:
- Apoptosis and Autophagy
- Cancer signaling
- Cardiology and bone biology
- Inflammation and Immunology
- Metabolism and Endocrinology
- Stem Cells
- Toxicity and Drug Metabolism
Download the QGP Pathway Panel Guide here for the complete list of predesigned panels.
eBioscience launched new Isotyping panels for mouse and rat:
ProcartaPlex Mouse Antibody Isotyping Panel 2 (IgG2c included) (7 plex) Certain inbred mice strains such asC57Bl/6, C57Bl/10, SJL, and NOD with the Igh1-b allele do not have the gene for IgG2a, but instead express IgG2c. Therefore, eBioscience included IgG2c into their new ProcartaPlex Mouse Isotyping Panel 2. Analytes: IgG1, IgG2b, IgG2c, IgG3, IgA, IgE, IgM
ProcartaPlex Rat Antibody Isotyping Panel (6 plex) Analytes: IgG1, IgG2a, IgG2b, IgG2c, IgA, IgM
More new products of eBioscience:
Non-Human Primates (NHP)
ProcartaPlex NHP Cytokine/Chemokine/Growth Factor Panel (37 plex) Analytes: BDNF, BLC (CXCL13), NGF beta, Eotaxin (CCL11), FGF-2, G-CSF (CSF-3), GM-CSF, IFN alpha, IFN gamma, IL-1 beta, IL-10, IL-12p70, IL-13, IL-15, IL-17A (CTLA-8), IL-18, IL-1RA, IL-2, IL-23, IL-4, IL-5, IL-6, IL-7, IL-8 (CXCL8), IP-10 (CXCL10), I-TAC (CXCL11), MCP-1 (CCL2), MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), PDGF-BB, sCD40L, SCF, SDF-1 alpha (CXCL12a), TNF alpha, VEGF-A, VEGF-D
ProcartaPlex NHP Cytokine & Chemokine Panel (30 plex) Analytes: BLC (CXCL13), Eotaxin (CCL11), G-CSF (CSF-3), GM-CSF, IFN alpha, IFN gamma, IL-1 beta, IL-10, IL-12p70, IL-13, IL-15, IL-17A (CTLA-8), IL-18, IL-1RA, IL-2, IL-23, IL-4, IL-5, IL-6, IL-7, IL-8 (CXCL8), IP-10 (CXCL10), I-TAC (CXCL11), MCP-1 (CCL2), MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), sCD40L, SDF-1 alpha (CXCL12a), TNF alpha
ProcartaPlex NHP Growth Factor Panel (7 plex) Analytes: BDNF, FGF-2, NGF beta, PDGF-BB, SCF, VEGF-A, VEGF-D Human
Human Myokine Panel (8 plex) Analytes: BDNF, IL-6, IL-8, IL-10, IL-15, IL-1RA, LIF, TNF alpha Mouse
Mouse Myokine Panel (5 plex) Analytes: IL-6, IL-10, IL-15/IL-15R, LIF, TNF alpha
For the first time ever, the most popular biomarkers of inflammation from the TNF superfamily proteins, IFN family proteins, Treg cytokines, and MMPs can be measured in a single multiplex kit. Available in larger 24- and 37-plex screening panels and a pathway-focused Treg 12-plex panel, along with singleplex and custom configured assays. Benefits of Bio-Plex Pro Inflammation Assays include:
- Unique and Relevant Mix of Targets—The only Luminex kit available that combines TNF superfamily proteins, IFN family proteins, Treg cytokines, and MMPs in one assay
- Flexible Ordering Options—Two large plex inflammation screening premixed ready to use kits (37 and 24 plex), a Treg 12-plex, singleplex, and any combination of custom kits available as “you mix” and “we mix” options
- Rapid results—Multiplex data in just 3.5 hours through the use of magnetic beads and the fastest assay protocols
Join us on November 6th for an informative webinar: “Biomarker Profiling in Diabetes and Cardiovascular Disease.” Learn how diabetes and related metabolic conditions are linked to cardiovascular disease, which protein biomarkers are currently being studied in these conditions, and how to leverage multiplex metabolic and hormone assays to profile multiple protein biomarkers relevant to metabolic and cardiovascular diseases.
EMD Millipore continues to provide the best, most relevant biomarkers on magnetic bead format:
- MILLIPLEX® MAP Non-Human Primate Cytokine Panel 2 – Configurable 25-Plex ( PRCYT2MAG40K), 24-Plex Premixed (PRCYTMAG40K-PX24), 25-Plex Premixed (PRCYTMAG40K-PX25)
- MILLIPLEX® MAP Mouse Cardiovascular Panel 2 – Configurable 9-Plex (MCVD2MAG-77K)
- MILLIPLEX® MAP Canine Kidney Toxicity Panel 2 – Configurable 4-Plex (CKT2MAG-97K)
- MILLIPLEX® MAP Rat Liver Injury Configurable 5-Plex (RLI1MAG-92K)
- MILLIPLEX® MAP Rat Adipocyte Configurable 7-Plex (RADPCMAG-82K)
- MILLIPLEX® MAP Rat Adipokine Configurable 7-Plex (RADPKMAG-80K)
- MILLIPLEX® MAP Rat Adiponectin Single Plex Kit (RADPNMAG-81K-01)
For a complete list of our offering, download the MILLIPLEX® MAP Analyte Quarterly! For powerful multiplexed biomarker analysis, count on the experts!
The Power of Biomarker Analysis
After years of gathering valuable tips and tricks, the multiplex assay experts at EMD Millipore have made them available to you–with our technical guide: “The Power of Biomarker Analysis.”
WEBINAR, November 4, 2014: Novel Multiplexed Assay Panels to Detect Kidney Injury Biomarkers in Mouse Models. Learn to:
- Use biomarkers in drug development and safety assessment
- Develop informative and predictive experimental models of kidney injury
- Successfully perform multiplexed immunodetection assays
Novex® multiplex assays enable the simultaneous analysis of multiple proteins in single samples from a broad range of biological sources. Based on Luminex xMAP (multi-analyte profiling) technology, Novex multiplex assays combine the efficiencies of multiplexing with the accuracy, sensitivity, reproducibility, and simplicity of ELISA. www.lifetechnologies.com/immunoassayguide
Webinar (Recorded from September 25, 2014): Biomarker Discovery, Quantitation, and Analysis with Multiplex Immunoassays. Duration: 60 minutes.
NeuroMAP™ v. 1.0 is a quantitative, multiplexed immunoassay service measuring 36 blood-based proteins associated with neurodegenerative disorders including Alzheimer’s disease, Parkinson’s disease, and Multiple Sclerosis. Built on Myriad RBM’s robust Multi-Analyte Profiling (MAP) technology, NeuroMAP can be used as a targeted proteomics approach to help identify and characterize novel biomarker candidates for neurodegenerative drug and diagnostic development programs. Visit our website to see a list of the biomarkers included.
Watch this webinar to explore:
- The rationale for early detection of Pulmonary Fibrosis
- Phenotypic characteristics of Subclinical Interstitial Lung Disease (ILD) in at risk populations
- A strategy to select blood biomarkers for Subclinical ILD
- How our translational research studies can improve the diagnosis and treatment of ILD
ProtATonce launches turnkey solutions for high-throughput screening (HTS). Assays for bulk use are offered via our assay development service for 500+ proteins and includes all buffers to run hundreds of plates. Our assays guarantee best performance since ProtATonce has screened 1000s antibodies and has selected the ones with maximum affinity and minimal cross-reactivity.
ProtATonce also offers an A-to-Z service that includes: cell culture, sample prep, data acquisition, and data analysis. Our A-to-Z service includes Systems Pharmacology for drug discovery that allows researchers to construct signaling pathways, identify drug mode of action, predict efficacy/toxicity and identify optimal target using Luminex assays.
R&D Systems, a Bio-Techne Brand
New Ordering Tool for Luminex Assays! We have a streamlined website tool for customizing Luminex multi-analyte assays. The tool walks you through all the important choices in designing the best assay for your research.