How Well Do You Know Your Sample Probe?
Some of the most common hardware problems that occur in the field are related to the sample probe. Is your instrument failing calibration or verification? Are you getting low bead count on your assay results? This could be due to the probe height adjustment, a clog, or a bent probe. Luckily, most of these issues can be solved through troubleshooting procedures performed by you, the customer.
A benefit to the MAGPIX and FlexMAP 3D systems is the Auto Height Adjustment function within the hardware and software. This allows you to adjust the probe height with just a click of a button and for each plate height to be saved for future runs.
Below you will find common symptoms and troubleshooting procedures for incorrect probe height and clogged/bent probes for all instruments. Be sure to check out the troubleshooting videos below!
|Probe Height Troubleshooting|
|Incorrect Probe Height Symptoms||Probe Height Troubleshooting|
|Sample Empty ErrorsSlow Event Rate
Air In the System
|For Luminex 100/200 Place the appropriate alignment tool in the selected well of the plate and retract:
Loosen the screw gently on the probe holder. Push probe down until the probe comes in contact with the plate. Tighten the screw.
|Clogged/Bent Probe Symptoms||Clog Troubleshooting|
|Slow Event RateCalibration/Verification failure
Sample Empty Errors
Fluid Dripping from the Probe
Large Pocket of Air at top of Syringe
|For Luminex 100/200 & FlexMAP 3D Remove the probe and sonicate narrow end in DI water for 2-3 minutes.
Flush DI water through probe from the narrow end out through the larger end.
Replace probe and verify probe height.
Run 3 back flushes, 3 drains, 2 alcohol flushes, and 3 washes.
For Bent Probe
The videos below will help guide you through adjusting the probe height and processing the sample probe bypass on the Luminex 200 Instrument.