High resolution microscopy and flow cytometry to advance your discovery
Amnis® ImageStream®X Mk II Imaging Flow Cytometer
The revolutionary ImageStream®X Mk II Imaging Flow Cytometer combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. This unique combination enables a broad range of applications that would be impossible using either technique alone.
The ImageStreamX Mk II System is a benchtop, multispectral, imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. By collecting large numbers of digital images per sample and providing a numerical representation of image-based features, the ImageStreamX Mk II combines the per cell information content provided by standard microscopy with the statistical significance afforded by large sample sizes common to standard flow cytometry. With the ImageStreamX Mk II System, fluorescence intensity measurements are acquired as with a conventional flow cytometer; however, the best applications for the ImageStreamX Mk II take advantage of the system’s imaging abilities to locate and quantitate the distribution of signals on in or between cells.
Download the Amnis Imaging Flow Cytometers brochure.
Machine Learning for IDEAS® 6.3
Luminex has released the latest version of the Amnis® IDEAS® 6.3 image analysis software, which now comes with the ability to activate the Machine Learning Module!
Machine Learning simplifies analysis by making it easier to classify cells based on specific parameters. To do this, the Machine Learning Module creates a unique classifier that helps researchers customize image-based analysis to their specific application—without the need for complex masking or feature generation. With Machine Learning, you can simply click on a cell of interest and the software will identify other cells with the same features.
In the example to the right, Machine Learning for IDEAS 6.3 was used to create three new classifiers to identify the overall shape of red blood cells in the dataset. Round, sickle, and sideways cells can be clearly enumerated using these three unique classifiers.
Featured ImageStreamXMk II Video
Watch how imaging in flow has changed research at Roswell Park Comprehensive Cancer Center (RPCCC).
At the RPCCC Flow and Image Cytometry facility, Drs. Paul Wallace, Hans Minderman, and staff provide users with the best tools available for increasing statistical power and throughput for applications like measuring NFkB translocation that were formerly performed by laborious microscopy. Watch to learn how Amnis protocols have helped RPCCC researchers detect rare events like circulating tumor cells.
Fast: Analyzes Thousands of Cells Per Second
The ImageStreamX Mk II Imaging Flow Cytometer can analyze up to 5,000 cells/sec with real-time intensity compensation — ideal for rare cell analysis.
Easy-to-Use: Simple User interface
The intuitive user interface provides real-time plotting and graphical gating, as well as images of every cell. The easy to use compensation wizard quickly guides you through setup of multi-color compensation matrices.
Flexible: Fits Your Sample, Your Targets, Your Fluorophores
The ImageStreamX II Imaging Flow Cytometer accepts up to 7 lasers and works with sample volumes of 20-200 ?L for added experimental flexibility — perfect for multi-user laboratories.
Efficient: Less Sample Volume, Less Sample Waste
Fluidics that provide up to 95% sample utilization when every cell is required. Recover unused sample for further analysis when orthogonal methods are required.
Adaptable: Any Upgrade, Any Time
The modular design of the ImageStreamX Mk II Imaging Flow Cytometer allows the system to grow with your research needs and budget through a broad range of field-installable options. Most options, including additional lasers, can be installed at the factory or upgraded in the field by Luminex-certified field service personnel.
Imaging Flow Cytometers – Research Areas
The powerful combination of quantitative image analysis and flow cytometry in a single platform creates exceptional new experimental capabilities. The following are examples of areas where the ImageStreamX Mk II and FlowSight Imaging Flow Cytometers can bring more power and insights into your research.
Stem Cell Biology
Application: Nuclear Translocation of NFkB
The analysis of NFkB nuclear translocation shown below used three lasers and eight of 12 available detection channels. Cells were phenotyped using fluorescently-labeled antibodies against CD14, CD45, CD16, and CD3, while two additional image channels were used to quantitatively assess nuclear localization of NFkB by cross-correlation of the nuclear (DAPI) and NFkB (FITC) images. Brightfield and darkfield (SSC) images of each cell are included to provide morphological and structural detail.
Optimized kits for imaging flow cytometry are available to measure nuclear translocation either with your own antibody or with anti-NFkB. In addition, given the ease of detecting several markers simultaneously, this system opens the door to multiplexing and evaluating activation of subpopulations in complex biological samples.
Amnis ImageStreamX Mk II Imaging Flow Cytometer Options
An Expandable Fluorescent Palette
The standard 488 nm laser of the ImageStreamX Mk II Imaging Flow Cytometer may be augmented with up to six additional lasers, including 375, 405, 561, 592, and 642 nm. A high-power 488 nm laser upgrade is also available for enhanced detection of fluorescent dyes excited at that wavelength.
- Increase experimental flexibility by utilizing a broader palette of fluorescent markers
- Improve data quality by employing more spectrally distinct dyes, thereby reducing crosstalk
- Improve throughput via multiplexing of experiments in a single tube or well
- Additional laser options can be installed in the field by certified technicians
Lasers and Example Dyes
See the Amnis Support page for Fluorofinder application.
AutoSampler for Multiwell Plates
The AutoSampler option for the ImageStreamX Mk II Imaging Flow Cytometer enhances productivity with unattended sample loading from multiwell plates. The fully integrated AutoSampler option allows you to easily perform dose response and time course studies even in primary samples, due to the system’s capacity for imaging large numbers of cells from each sample.
- Ideal for high throughput analysis of suspended cells
- Easy to use acquisition templates allow for multiple experiments per plate
- Unattended operation and in-line bubble detection enables robust operation
- Automatic process logging and error notification
- Automatic sipper rinse between samples and <0.5% carryover
- Automatic sample resuspension via aspiration
- Advanced template function allows groups of wells to be defined for flexible plate configuration
- Automated data analysis
- Field installable by certified technicians
12 Image Channels
Up to 12 Images of Every Cell
The 12 Image Channel option significantly expands the experimental flexibility and analytical potential of the ImageStreamX Mk II Imaging Flow Cytometer by doubling the number of images per cell from six to twelve. This option is comprised of a second image detection system, including a filter wheel, inline filters, spectral decomposition assembly, and a proprietary charge-coupled device (CCD) camera.
- Increase experimental capacity by utilizing a broader palette of fluorescent markers
- Improve data quality by reducing or eliminating spectral crosstalk between detection channels
- Perform functional assays of immunophenotypically-defined subpopulations
- Improve throughput via multiplexing of experiments in a single tube or sample well
- Field installable by certified technicians
The MultiMag option provides 20X and 60X objective lenses, in addition to the standard 40X objective, for greater experimental flexibility and improved resolution. The 60X objective lens increases magnification for small objects, such as yeast and bacteria, and offers greater detail with mammalian and plant cells. The 20X objective lens provides an increased field of view for the imaging of large cells, such as epithelial cells.
- Superior quality, high numerical aperture (NA) objectives yield crisp images
- Convenient magnification changes via motorized objective mount
- Crisp focus is ensured by advanced, patented auto focus technology
The Right Magnification for Your Work
60X magnification for higher resolution
20X magnification for large cells
Extended Depth of Field
A unique optical system upgrade for the ImageStreamX Mk II Cytometer keeps the depth of cell in focus without loss of fluorescence sensitivity. Now you can image FISH spots or other sub-cellular features, no matter where they lie in the cell.
Image more of the cell in focus
The extended depth of field (EDF™) option for the ImageStreamX Mk II Cytometer incorporates Wavefront Coding™ technology from CDM Optics, which is a combination of specialized optics and unique image processing algorithms, to project more structures within the cell into one crisp plane of focus.
EDF technology dramatically extends the depth of field, enabling new applications and improving the performance of existing applications – especially spot counting applications such as FISH, autophagy, and nuclear translocation.
Upgrade EDF package includes specialized optics and image processing algorithms
- Projects all features of a cell into a single plane of focus
- Enables precise analysis of fluorescence in situ hybridization in suspension (FISHIS)
Machine Learning for IDEAS® 6.3 – Flyer
Imaging Flow Cytometry of Mixed Algae Cultures for Biomass Evaluation – Application Note
Luminex flow cytometers and cellular analysis instruments give you instant access to all facets of cellular phenotypes and morphology.