Guava® Software

Guava® InCyte™ and Guava® Suite Software Configuration

Your specific research needs are always changing, and Luminex’s Guava® optimized software modules are flexible enough to accommodate you at every level.

The Guava Suite Software Package consists of a choice of two acquisition and analysis platforms:

• Guava InCyte Software Package enables easy visualization of data in a broad biological context.
• Modules available for assays relating to count and viability, cell cycle, mitochondrial potential, apoptosis, and TUNEL.

The Guava Suite Software Package provides an intuitive user interface and offers acquisition, instrument set-up, and analysis capabilities in each dedicated software module. You can export to spreadsheets or any third-party analysis format. 21 CFR part 11 enabling features are included with specific application modules. Guava InCyte Software includes features that can be applied to control user access to data files and methods, which is useful in multi-user environments. The Guava Suite Software Package also includes easyCheck™ for convenient QC of the instrument and a cleaning function to automate cleaning routines.

Guava InCyte

Guava InCyte is a flexible software package designed to allow the advanced user to set-up and analyze an assay quickly, offering unique tools for rapid data comparison and visualization.

Guava Suite

Designed for ease of use. Modules use an interface which enables easy sample acquisition and are optimized for each assay.

Guava InCyte and Guava Suite Software Features

Guava InCyte Software

Guava InCyte Software is the first analysis package designed specifically to give every user the power to draw conclusions about the biological significance of data. It has an intuitive, easy to use interface that makes it possible to visualize and compare up to eight data sets at the same time. The software is ideally suited for interrogation of high content data sets derived from multiple functional studies commonly employed during compound screening and target identification. Guava InCyte brings a new level of analytical power to flow cytometry, enabling users to analyze an entire plate of data. In addition, data acquisition is fully incorporated so that it can function as the primary data acquisition and analysis package for the instrument. Most importantly, comparative results are displayed at the experiment level rather than on an individual well/sample basis.

Features

• Drag and drop gating from one plot to another
• View up to 20 plots at once
• Real-time plot adjustments
• Organize acquired data sets and select individual wells for display
• Easily create analysis templates
• Quickly link to and review previously analyzed data
• Combine groups of data and analysis templates
• Slider bars set cut-offs or threshold values for each experimental sector
• Heat map shows values across an entire plate
• Easily generate IC₅₀/EC₅₀ curves within the software

For more information about the Guava InCyte Software, read our software application note here.

Guava InCyte Software Heat Map and IC50/EC50

Hela cells in microtiter plates were treated with multiple cytotoxic compounds for 24 hours. Cells were stained using FlowCellect® MitoDamage, MitoCaspase, or MitoStress kits. Cells were analyzed on the Guava platform and percent population data were compared in a heat map format using Guava InCyte Software. Guava InCyte Software allowed for the quick identification of hit compounds and comparison of all 5 parameters simultaneously as shown in the pie charts above. The data above shows the data for the 80 compounds on both cell lines.

IC50 determination using the Cytochrome c Kit and analyzed the built-in IC-50/EC-50 curve fitting feature of Guava InCyte. Cells were analyzed on the Guava easyCyte 8HT. Plot A shows the drag and drop gating strategy used for the IC50 determination. Plot B shows the IC50 curve results for gambogic acid and Plot C shows the EC50 for etoposide. The once complex task of finding the IC50 or EC50 for a compound has been made as easy as a few clicks of the mouse.

Guava Suite Assay-specific Software

Assay-specific Software Modules: Optimized

Get to the most meaningful data more quickly. Optimized for each assay, Guava assay-specific software modules display only the plots and statistics you need. Each software module acquires and analyzes data, then exports results to a database without additional user intervention. This integrated, automated process makes your cell analysis both easier and more accurate.

Features

• One-click acquisition and analysis
• Pre-set plot templates specific to assay
• Friendly interface with step-by-step guide
• Assay results with statistics automatically calculated and displayed to minimize error
• Sample volume tracking
• Accurate cell counts without reference beads

InCyte and Guava Suite Software Videos

The GuavaSoft™ System includes templates for use with a wide range of applications to simplify data acquisition and analysis. The videos below show setup of instrument experimental parameters, as well as acquisition and data analysis using application-specific modules or the open-application Guava InCyte Software. With these tutorials and our optimized reagents, instrument calibration and precise cell count and viability assessment are a snap. Step-by-step demonstrations illustrate how GuavaSoft helps you effortlessly generate EC50/IC50 curves to measure compound potency and toxicity, and our unique heatmapping feature enables analysis of entire multiwell plates in the time normally required to assess a single sample.