Our microcapillary flow cytometry systems are simpler to operate than traditional sheath-fluid based instruments and are far easier to maintain. They utilize small sample volumes, generate minimal waste, and have lower operating costs. As a result, easyCyte™ Flow Cytometers are uniquely amenable to on-demand use in the laboratory environment and have helped many scientists achieve insightful cellular analysis since 1998.
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Choose the optical and sample handling configuration that best suits your research needs.
|Excitation Laser||405 nm||488 nm
|532 nm||642 nm|
|5HPL, 5HPL HT||✔|
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Single, dual, or triple excitation laser configurations provide up to 14 simultaneous detection parameters, including 12 fluorescent colors plus forward and side scatter for size and granularity determination. For applications requiring exceptional sensitivity, a high-power blue laser (HPL) is offered in both single- and HT-loader formats.
easyCyte Flow Cytometers use novel microcapillary technology that enables a compact user-friendly format, which is powerful enough to run your most complex assays. These simple fluidics enable a self-aligning flow cell that can be removed for cleaning by the user. New, more robust lasers available in virtually any combination mean that you choose the excitation and emission channels that are a match for your lab’s experiments and budget. All of our versatile optical configurations are available with an affordable single-sample loader, or an integrated multiwell sample tray option, for trouble-free walkaway acquisition of up to 96 samples.
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All easyCyte Systems use a unique, microcapillary flow cell for easy operation and minimal maintenance
Direct absolute counts without reference beads
Absolute cell counts are a distinct advantage of direct sampling, and automatic cell enumeration is returned from every sample acquired on an easyCyte Cytometer. A positive displacement syringe pump enables not only direct sampling, but can adjust the flow rate as well to ensure clear data from a range of sample titers.
Automated Sampling for 96-well Plates
The easyCyte HT series enables walkaway sample handling for higher throughput assays using a built-in, automated 96-well plate loader tray, which can hold a 96-well plate as well as 10 sample tubes. To ensure that cells stay in suspension during sampling, a rotating, variable- speed mixing paddle with auto-cleaning is used between each well so there is no need for manual resuspension after loading the plate. This virtually eliminates carryover between samples and mixes more efficiently than shaking plate holder formats.
Single Sample Loading for Tubes
For manual sample handling in a cost-effective format, we offer the easyCyte Instrument with its rotating sample tube loader. The manual loader holds two separate tubes, allowing the user to progress quickly from one sample to another, or to wash between samples following an acquisition.
Thoughtful laser and detector selection ensures that easyCyte Instruments are compatible with familiar and trusted fluorophores, as well as many of the newer dyes that offer advantages such as increased brightness or photostability.
The easyCyte format is compact and offers many new innovations in optical performance and software.
Accuracy on the easyCyte 8HT is shown using a human whole blood cocktail (FlowCellect® Human CD4/CD8 T Cell Kit). Results were comparable to a predicate method on a sheath-fluid based flow cytometer.
Accuracy on the easyCyte 8HT is comparable to traditional flow cytometry
Superior small particle bead detection down to 0.2 μm now on the easyCyte 12 instruments
To ensure that cells stay in suspension during sampling, a rotating, variable-speed mixing paddle with auto-cleaning is used between each well so there is no need for manual resuspension after loading the plate.
The mixing feature virtually eliminates carryover between samples and mixes more efficiently than shaking plate holder formats. Data shown below confirms that carryover is minimized with this format.
|Plate 1||Plate 2||Plate 3||Plate 4||Plate 5|
|Carryover||0.15% ±||0.17% ±||0.21% ±||0.23% ±||0.22% ±|
The easyCyte 12HT System accurately discriminates eight different fluorochromes within a single assay.
Guava Suite Software for acquisition and analysis is highly flexible and user-friendly. For more information on our software, click here.
Every easyCyte Instrument has optimized calibrated performance to ensure instrument to instrument reproducibility. This means that there is no user adjustment necessary.
The ability to transfer instrument settings and methods between instruments allows comparisons of data between instruments and cross-site validation studies.
The easyCheck Kit is used to verify system performance and maintain daily quality control before running any assays, and is superior to instrument calibration for this purpose. The kit consists of easyCheck standardized fluorescent beads and diluent, facilitating assessment of counting accuracy and fluorescence detection. The easyCheck Kit is included with all instruments to enable daily, routine signal optimization and quality control.
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Since 1998, Guava systems have helped many scientists achieve insightful cellular analysis.
Luminex flow cytometers and cellular analysis instruments give you instant access to all facets of cellular phenotypes and morphology.
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