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VERIGENE® NANOGRID TECHNOLOGY

Verigene® Technology

The proprietary gold nanoparticle probe technology is the driving force behind the accuracy and cost-effectiveness of all Verigene tests

About Verigene Technology

 

The Verigene® System utilizes advanced automation and proprietary chemistry to enable rapid direct detection of nucleic acids and high-sensitivity protein detection on the same platform. The unique gold nanoparticle probe technology is the driving force behind all Verigene tests, providing a versatile and extremely reliable foundation for the Verigene System’s growing menu of clinically meaningful diagnostics.

 

Nanoparticles

 

Nanoparticles, as used in the Verigene® System, are typically 13-20 nanometers (nm) in diameter. One nanometer is approximately one ten-thousandth (1/10,000) of the width of a human hair.

 

Depending upon the application, each nanoparticle is functionalized with either a defined number of oligonucleotides (i.e., short pieces of DNA or RNA) or a defined number of antibodies that are specific to a particular protein of interest.

 

 

Gold Nanoparticle Technology

 

Gold nanoparticle probes possess several properties that make them ideal for diagnostic applications:

  • Allow increased sensitivity by several orders of magnitude compared to fluorophores; light scattered from one nanoparticle is equivalent to the light emitted from 500,000 fluorophores
  • Enable high-specificity for both nucleic acid and protein detection
  • Reduce background noise, which in turn, creates an enhanced assay signal
  • Are extremely stable, have a long shelf-life, and are non-toxic

The Chemistry

 

Direct Detection of Nucleic Acids

 

The detection of DNA or RNA targets using nanoparticle probe technology entails:

  • Automated nucleic acid extraction and PCR amplification (if necessary) from a clinical sample on the Verigene® Processor SP
  • Automated transfer of eluted nucleic acids into a Verigene Test Cartridge for hybridization
  • Primary hybridization of target DNA to capture oligonucleotides on a microarray
  • Specific mediator oligonucleotides and gold nanoparticle probes
  • Signal amplification of hybridized probes via a silver staining process
  • Automated qualitative analysis of results performed on the Verigene Reader

 

High-Sensitivity Protein Diagnostics

 

Ultra-sensitive detection of protein targets, using nanoparticle probe technology, entails:

  • Introduction of serum from a clinical sample into a Verigene Test Cartridge
  • Capture of protein(s) of interest from the sample via simultaneous hybridization to:
    • Primary antibodies specific to the protein of interest fixed in a microarray format
    • Gold nanoparticle probes loaded with secondary antibodies specific for the protein of interest
    • Signal amplification of hybridized probes via a silver staining process
    • Automated (qualitative or quantitative) analysis of results performed on the Verigene Reader