There are currently more than 60 Luminex Licensed Technologies Partners worldwide that provide kits, assay development, and testing services for the research and diagnostic markets. While you can find more than 1,400 commercially available multiplex immunoassays in the xMAP® Kit Finder, below is a selection of the newest products and services.
MILLIPLEX® MAP Mouse High Sensitivity T Cell Panel
Low levels of inflammation are involved in many clinical and subclinical disease states. Measuring picogram levels of cytokines is critical for understanding the pathogenesis of these diseases, particularly in model organisms like mice. Cytokines and chemokines function as biomarkers for various diseases such as:
- Autoimmune disease
- Cardiovascular disease
- Neurological disorders
Design your multiplex kit by choosing available analytes within the MILLIPLEX® MAP Mouse High Sensitivity T Cell Panel: GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 (p70), IL-13, IL-17A, KC/CXCL1, LIX, MCP-1, MIP-2 and TNF-α.
- MILLIPLEX MAP Mouse High Sensitivity T Cell Panel: MHSTCMAG-70K
- MILLIPLEX MAP Mouse High Sensitivity T Cell Premixed Panel: MHSTCMAG-70KPMX
- MILLIPLEX MAP Mouse High Sensitivity T Cell Panel- Premix Bulk (Space Saver) Packaging: MHSTCMAG-70KPXBK
Plasma samples from an LPS-challenge in vivo mouse time course experiment showed a spike in analyte concentrations over the course of the study in multiple analytes (M-CSF, IL-1β, IL-2, and IL-5 are shown below).
Bio-Plex™ Pro Human Apolipoprotein Panel
Apolipoproteins are important proteins that serve many functions. They bind to lipids and transport them through the lymphatic and circulatory systems. Apolipoproteins also serve as enzyme cofactors, receptor ligands, and lipid transfer carriers that regulate lipoprotein metabolism and their uptake in tissues.
Apolipoproteins also function as biomarkers for various conditions such as:
- Cardiovascular diseases
- Liver Disease
Detecting changes in biomarker levels or the presence of biomarkers such as apolipoproteins can be difficult with traditional ELISA techniques. Multiplex solutions offer higher sensitivity and conserve precious sample by testing for multiple analytes in a single well.
While there are several multiplex options available, the new Bio-Plex Pro™ Human Apolipoprotein Panel allows you to rapidly and efficiently detect nine key apolipoproteins plus C-Reactive Protein (CRP) from 10 μL of sample in a single well in less than 4 hours
The Human Apolipoprotein Panel enables the detection and quantification of the following ten analytes in human biological samples: Apo A1, Apo A2, Apo B, Apo C1, Apo C3, Apo D, Apo E, Apo H, Apo J, and CRP (C-Reactive Protein).
Bio-Plex Pro™ Human Apolipoprotein Assay Panel, 10-plex 12003081
Go to www.bio-rad.com/NewApo to learn more
R&D Systems, a Bio-Techne Brand
High Performance Assays 101: The Devil is in the Diluents
Assay diluents matter because simultaneous detection of multiple protein analytes presents many challenges. This includes assay non-specific binding, cross-reactivity, and antibody interference. R&D Systems recognizes these unique difficulties. R&D Systems recognizes these unique difficulties. The diluents provided with R&D Systems’ Luminex High Performance Assays have been tested and specifically formulated to overcome the challenges listed above that are unavoidable in multiplex assays.
Defining Interference, Non-specific Binding, and Cross-reactivity
Reagents provided in R&D Systems’ Luminex Assays are carefully designed and tested to minimize assay interference and ensure an optimally functional sandwich immunoassay (Figure 1). Immunoassay interference occurs when a substance within the assay prevents the accurate detection of the target analyte. It can occur due to non specific antibody binding, antibody cross-reactivity, or antibody interference. In addition, complex sample matrices, such as serum and plasma, can often contain interfering factors.
Interfering factors may also be introduced to biological samples via the reagents or equipment used. Interference, no matter the mechanism, can result in either reduced or elevated signal relative to the actual concentration of target analyte. This means untrustworthy data. Read below to learn how we carefully design our Luminex High Performance Assay diluents to overcome these challenges, ensuring that you can always trust the data you generate.
Non-specific binding occurs in an immunoassay when the antibody pair interacts with the sample container or other assay surfaces and contributes to background (Figure 2). Non specific binding to microparticles, microplates, or other assay surfaces can be reduced by blocking. Diluents provided with R&D Systems’ Luminex High Performance Assays contain assay optimized blocking reagents to ensure low non-specific binding
Cross-reactivity, the interaction of the antibody pair with a molecule other than the targeted analyte, can be caused by either the capture antibody or the detection antibody and often occurs when proteins in the sample are structurally similar to the analyte of interest (Figure 3). Optimizing antibodies so as to avoid crossreactivity with substances within the biological sample is often challenging. R&D Systems carefully selects and exhaustively tests our in-house developed antibody pairs used in our Luminex High Performance Assays to ensure analyte specificity
Antibody interference occurs when endogenous antibodies within samples cross-link with assay antibodies (Figure 4; A-C) or substances within the sample (Figure 4; D, E) and prevent proper target-analyte binding to both the capture and detection antibodies. Common antibody interfering substances in biological samples include human anti-mouse antibodies (HAMA) and rheumatoid factor (RF) antibodies, as well as any substance present in exceptionally high concentrations. Diluents provided with our Luminex High Performance Assays are designed to prevent antibody interference from HAMA and RF in biological samples.
Learn more and design your Luminex High-Performance assay at https://www.rndsystems.com/products/luminex-high-performance-assays
Thermo Fisher Scientific
Did you know that you can analyze both protein and gene expression from a single sample?
Protein and gene expression data can be generated from the same sample, giving researchers more information about that sample. After a cell culture is centrifuged, the supernatant is analyzed for multiple cytokines using the Invitrogen™ ProcartaPlex™ assay. The cell pellet is then lysed and run using the Invitrogen QuantiGene™ Plex assay to obtain gene expression data.
Contact [email protected] for personalized assistance and to get started.