Ultra-high sensitivity and flexibility
High Sensitivity Flow Cytometry Enables Extracellular Vesicle Immunophenotyping
The Amnis® CellStream® benchtop flow cytometry system from Luminex is a highly-customizable and compact flow cytometer that is the first to use a camera for detection. Only recently has the importance of extracellular vesicles as key mediators of intercellular communication been appreciated. Watch this presentation to learn more about how the unique Amnis® time delay integration and camera technology inside the CellStream System enables immunophenotyping of extracellular vesicles. (33 min)
CellStream Benchtop Flow Cytometer for Sensitive Cell Analysis
- Unparalleled Sensitivity—The CellStream Flow Cytometry System delivers high fluorescence sensitivity with MESF <10 for FITC and MESF <5 for PE.
- 1 to 7 Laser Capacity—The CellStream System can be configured with 1 to 7 lasers. Laser upgrades are easily performed in the lab with no downtime.
- Unique Event Gallery Software Feature—This unique camera-enabled feature of the CellStream Acquisition Software allows for population verification, aids in troubleshooting, and resolves doublets.
- Standard 96-well Plate AutoSampler—All CellStream Systems are equipped for both single tubes and 96-well plates. On-board fluidics handle all mixing and rinsing steps to give you walkaway, high throughput acquisition.
- Patented Optics Technology—The CellStream Flow Cytometry System contains patented time delay integration (TDI) and camera technology to rapidly capture low-resolution cell images and transform these into high throughput intensity data.
Excitation and Emission Capabilities of the 7-Laser CellStream Flow Cytometer
Sample Handling for Both Single Tubes & 96-Well Plates is Standard on All Instruments
The CellStream Flow Cytometry System enables walkaway sample handling for high throughput assays. The built-in, automated plate loader tray holds a 96-well plate and is a standard feature on all instruments. To ensure that cells stay in suspension during sampling, each well is mixed by the sample probe before sampling, so there is no need for manual resuspension after loading the plate. To prevent carryover, the sample probe is washed between samples with on-board fluidics. This virtually eliminates the risk of contamination between samples and results in more efficient mixing than shaking plate holder formats or aspiration methods.
CellStream Instrument Specifications
|Fluorescence Sensitivity||MESF* <10 FITC
MESF <5 PE
|Number of Channels||Up to 22 (20 fluorescent, plus FSC, SSC)|
|Number of Lasers||1-7|
|Available Lasers||375, 405, 488, 532, 561, 642, 730 nm|
|Camera-enabled Morphology Parameters||3 (area; aspect ratio; raw max. pixel)|
|Event Rate||20,000 cells/second|
|Flow Rates||3.66 µL/min (Low speed/High sensitivity)
14.64 µL/min (High speed)
|Scatter Resolution||FSC <300 nm from 450 nm
SSC <200 nm from 785 nm
|Dynamic Range||7 decades|
|System Size (W x D x H)||440 x 625 x 495 mm|
|Sample Formats||Single tube or 96-well plate|
|Absolute Cell Counting||Yes|
* Molecules of Equivalent Soluble Fluorochrome
** Coefficient of Variation using Chicken Erythrocyte Nuclei (CEN)
CellStream Optics Technology
A Flow Cytometry System with Patented Optics and a Highly Configurable 7-Laser Capacity
The CellStream Benchtop Flow Cytometer features patented Amnis® optics camera technology and the ability to configure the system with 1 to 7 lasers. This gives researchers unparalleled sensitivity, plus flexibility to customize and expand the system according to research needs and budget.
Compatible with Many Common Fluorochromes and Fluorescent Reagents
Thoughtful laser and detector selection ensures that CellStream instruments are compatible with familiar and trusted fluorophores, as well as many of the newer dyes that offer advantages such as increased brightness and photo-stability.
CellStream System Architecture
Our patented TDI and camera technology delivers sensitivity and expandability beyond what is possible with traditional flow cytometers:
- Up to 7 lasers are focused in discrete locations.
- Hydrodynamically focused cells pass through the laser-illuminated region. Fluorochromes bound to the cells are excited and emit into the collection system. Fluorescence is collected and directed toward an intermediate image plane.
- The filter stack decomposes each of the four discrete vertical positions in the intermediate image plane into 22 separate channels of data.
- All 22 channels fit efficiently onto a CCD (charge-coupled device) array.
CellStream System’s sensor contains multiple discrete collection fields using the same CCD as patented Amnis® technology
How CellStream Flow Cytometry Optics Work
CCD Camera in Time-delay Integration
CellStream Systems, like our state-of-the-art Amnis® flow cytometers, employ a TDI-CCD camera. This is the same technology used in medical radiography and military applications to capture images of moving objects.
The custom camera within CellStream Flow Cytometers operates using this TDI technique, whereby a specialized detector readout mode preserves sensitivity and image quality, even with fast relative movement between the detector and the objects being imaged. The TDI detection technology of the CCD camera allows up to 1,000 times more signal to be acquired from cells in flow than from conventional frame imaging approaches. Velocity detection and autofocus systems maintain proper camera synchronization and focus during the process of image acquisition.
As with any CCD, image photons are converted to photocharges in an array of pixels. During TDI operation, the photocharges are continuously shifted from pixel to pixel down the detector, parallel to the axis of flow, integrating the signal over multiple pixels. By synchronizing the photocharge shift rate with the velocity of the flowing cell, the effect is similar to physically panning a camera. With TDI, image streaking is avoided despite signal integration times that are orders of magnitude longer than those of conventional flow cytometry. Each pixel is digitized with 12 bits of intensity resolution, providing a minimum dynamic range of three decades per pixel.
CellStream Instrument Performance
A Flow Cytometry System with Patented Optics and a Highly Configurable 7-laser Capacity
The CellStream Flow Cytometer offers multiparameter detection capabilities and superior sensitivity in a compact, benchtop format. With the same patent-protected optical system as our state-of-the-art Amnis® instruments, CellStream Flow Cytometers rapidly capture low-resolution cell images and transform these into high throughput intensity data.
Download the CellStream application note here.
High Fluorescence Sensitivity: 8-peak Rainbow Beads
Fluorescence sensitivity is demonstrated below using industry standard 8-peak rainbow calibration beads. The CellStream Flow Cytometer clearly resolves all 8 peaks on every detection channel, with low MESF (Molecules of Equivalent Soluble Fluorochrome) values:
- MESF <10 FITC
- MESF <5 PE
Small, Submicron Particle Detection
Size bead data demonstrates superior detection and discrimination of small particles on the CellStream platform.
Superior Small Particle Detection: Size Bead Data
Acquisition of Megamix-Plus FSC size beads containing 300, 500, and 900 nm fluorescent beads in a known ratio of 4:2:1. Instrument settings: 70 mW SSC, 10% FSC, and 200 mW 488 nm; slow speed.
Immunological Phenotyping 10-color Assay
In this example, a 4-laser CellStream System accurately resolves 10 different fluorochromes within a single assay. The data below show how different immune cell populations are clearly resolved within a sample of PBMCs.
Immunophenotyping Extracellular Vesicles Using Amnis Technology – Application Note
Luminex flow cytometers and cellular analysis instruments give you instant access to all facets of cellular phenotypes and morphology.
Amnis ImageStreamX Mk II
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