An Expert’s Guide to Multiplexing Immunoassays

January 16th, 2018 / Christine Valle

New DD&D article looks at technical considerations

Andrew Hudacek is a power user of our xMAP® Technology for designing multiplex immunoassays. As the Custom Luminex Assay Supervisor at Bio-Techne®, he provides valuable expertise in assay design and validation to customers in pharma and biotech organizations.
Andrew Hudacek, Custom Luminex Assay Supervisor at Bio-Techne®

So we were delighted to see a new article from Hudacek in Drug Discovery & Development about best practices and technical considerations for designing multiplex immunoassays. It’s a great resource for anyone interested in developing these assays on their own.

In the article, Hudacek notes that “there is a growing need for sensitive immunoassays to assess clinically relevant markers of therapeutic response to a drug or immunotherapy, as well as markers of toxicity” throughout the drug discovery and development pipelines. “The need for so much testing has put pressure on drug discovery scientists to rein in costs and optimize sample usage by designing multiplex assays to cover several analytes simultaneously,” he adds.

Multiplexing benefits include dramatically lower costs, more judicious use of precious samples, and reduced hands-on time, Hudacek writes. But designing these assays can be a challenge, from selecting the right antibody pairs and optimal buffer to carefully validating the overall performance of each new assay. The article walks through each of those technical factors, offering readers useful tips to help in the lab.

Lab Testing

“Immunoassay developers strive for many performance goals—particularly specificity, sensitivity, precision, accuracy, and reproducibility,” Hudacek concludes. “Paying attention to the technical factors explored here will significantly improve the chances for developing high-quality and effective multiplex assays.”

Read the complete article in Drug Discovery & Development: Multiplexing Immunoassays: Technical Considerations for Optimal Performance.

For more information about designing your own assays, download our xMAP® Cookbook.

To view commercially available kits, visit our xMAP® Kitfinder.

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