Genetic tests from Luminex are based on xTAG® Technology, which utilizes a proprietary universal tag system that allows easy optimization, product development and expansion of molecular diagnostic assays. xTAG® technology combined with the Luminex instrument are a best in class solution for performing clinical multiplexed genetic tests.
I. Multiplex PCR
A multiplexed PCR reaction is performed to amplify the regions of interest in the target human or infectious agent genes.
II. Exo/SAP treatment of PCR Product The PCR reaction is treated to remove excess nucleotides and primers.
III. Multiplex ASPE/TSPE The PCR reaction is then subjected to a primer extension step that is specific for the allele or the infectious agent that is being analyzed (Allele Specific Primer Extension (ASPE) or Target Specific Primer Extension (TSPE)). The 5’ end of the ASPE or TSPE primers is attached to an xTAG® universal tag sequence.
IV. Universal Array Sorting The 5’ universal tag sequence is hybridized to the complementary anti-tag sequence coupled to a particular xMAP bead set.
Wash (Remove in Streamlined)
V. Detection The hybridized beads are read by the Luminex System and results are analyzed by the data analysis software.
Luminex System Detection
Luminex xTAG® products provide accurate, flexible, low cost microarrays, combining any set of 100 single DNA tests and multiplexing them simultaneously in a single reaction. xTAG® products are conducted on the Luminex xMAP® system, which uses lasers to read color-coded microspheres that attach to specific nucleic acid sequences. xTAG® was formerly known as TAG-IT from TmBioscience.
For In Vitro Diagnostic Use Only. ContactLuminex for more information.
Products listed are region specific and intended for residents of a particular country/region; this site contains information on devices that may not be approved in some countries/regions, therefore please contact Luminex at support@luminexcorp.com to obtain the appropriate product information for your country of residence.
Clinical Assays in Development
• xTAG® Ashkenazi Jewish Panel
• xTAG® Kit for Factor V,II and MTHFR 677/1298
• xTAG® Kit for CYP2C19
• xTAG® Kit for CYP2D6
• xTAG® Kit for CYP2C9+VKORC1
For Investigational Use Only. The performance characteristics of this product have not been established.
