|
Don’t want to buy a full array just to study a select set of microRNAs?
Don't like the multiplex limitations of qPCR?
Want same day results with quality data?
With the FlexmiR® v2 assay, you can build a multiplex assay with any microRNA sequence you want - up to 50 microRNAs in a single reaction!
One kit does it all!
- Test any miRNA Sequence *
- No Sample Labeling
- No Amplification
- Only 30 Minutes of Handling
- 1-50 Plex per Sample
- Results in Less than 5 hours
|
 |
Discover Powerful Simplicity
The FlexmiR v2 multiplex microRNA assay is an easy-to-use, highly sensitive and specific method for microRNA detection and quantitation. The assay can be performed from start to finish in 4-5 hours, with only 30 minutes of hands-on time.
Unlike other miRNA test methods, the FlexmiR v2 assay does not require sample modification such as biotinylation, dephosphorylation, fractionation, or amplification. A combination of highly specific hybridization plus enzymatic discrimination allows for a bias-free assay using Luminex’s powerful xMAP® technology.
Improved Chemistry
This next-generation FlexmiR product remains an xMAP liquid bead array, meaning you still get faster, more reproducible results than competing planar arrays, which are limited by solid-phase kinetics. Additionally, FlexmiR v2 offers:
- Better Specificity
- Greater Sensitivity
- Streamlined Workflow
- No Filter Plate Requirement (MagPlex® Bead Based)
- Attractive Pricing
Sensitivity
The FlexmiR v2 assay is designed to detect a minimum of 200 amols of a specific mature microRNA in a total RNA preparation, however users may observe much higher sensitivity (see below). This level of sensitivity is achieved with no enrichment or amplification.
Specificity
By combining a step-down hybridization with an enzymatic treatment, the FlexmiR v2 assay can discriminate betwwen closely related microRNAs, even those with single base differences near their terminus (see below)**. This specificity is not possible with most other assays.
* Mature microRNA sequences 16-35 bases in length
**Excludes single based G:U wobble
|
