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Upon administration of a pneumococcal vaccine, antibody response can achieve measurable concentrations in 2 to 5 weeks. The efficacy of the vaccination is generally assessed by measuring pre- and post-vaccination antibody titers. A 2-fold increase, or greater, is expected in most adults¹. Published literature considers that immunoglobulin G (IgG) response varies by serotype and can be impacted by age and health status¹ ². There is currently no documented agreement on specific post-vaccination antibody concentrations needed to establish protection from pneumococcal infection.
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Assay Description
The Luminex Pneumococcal Immunity Panel utilizes Luminex xMAP® multiplexing technology to provide a quantitative assessment of IgG following administration of a pneumococcal vaccine.
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xMAP® Pneumo14 contains the following pneumococcal serotypes:
| American Designation |
1 |
3 |
4 |
8 |
9 |
12 |
14 |
19 |
23 |
26 |
51 |
56 |
57 |
68 |
| Danish Designation |
1 |
3 |
4 |
8 |
9N |
12F |
14 |
19F |
23F |
6B |
7F |
18C |
19A |
9V |
Laboratory developed enzyme-linked immunosorbent assays (ELISA) are used by major reference laboratories and hospital laboratories to measure antibody response to vaccination. Each ELISA measures a single serotype on one plate requiring a total of 14 plates to test 14 serotypes. By contrast the xMAP® Pneumo14 assay allows for the simultaneous measurement of all 14 serotypes from a single patient sample in a single well. This significantly reduces the amount of labor and time to results versus traditional methods.
Comparison of xMAP® Pneumo14 to ELISA.
Inter-Laboratory Reproducibility
Since the assay is usually executed with pre- and post-vaccination samples, reproducibility is of utmost importance. This assay demonstrates superior reproducibility in the most stringent inter-laboratory scenarios. In this example, 80 blinded samples run at two different laboratories showed an average correlation coefficients of 0.96.
Median Correlation Coefficient r² = 0.97
Kit Contents
Microspheres coated with 14 pneumococcal serotype specific antigens
3 Controls: High (CH), Medium (CM), and Low (CL)
6 Standards and 1 Background
Conjugate
Wash Solution
Diluent
For more information about the Pneumococcal Immunity Panel, please contact info@luminexcorp.com
Order Information
| Product Description |
Product Number |
| xMAP® Pneumo14 (Ten Plate Kit) |
BG-PNEU00014-10 |
| xMAP® Pneumo14 (One Plate Kit) |
BG-PNEU00014-01 |
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