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Wednesday, April 27 - Partner Conducted Workshops
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LINCO Research
Workshop Title: Multiplexing in Metabolic Disease Studies: Factors That Influence a Robust Assay
Presenter: Sonali Nayak, LINCO Research, Inc.
Metabolic syndrome is characterized by several risk factors which include visceral obesity, atherogenic dyslipidemia, glucose intolerance, prothrombosis and inflammation. Individuals exhibiting these symptoms stand greater risk of coronary heart disease, atherosclerosis and diabetes. Hence studies done in this area cover multi-faceted fields of cytokines, acute phase proteins, diabetes and obesity related hormones and other cardiovascular disease biomarkers.
Multiplexed assays enhance the efficiency in generating information with speed, low sample volume and economy. This workshop describes such assays and discusses the factors that influence a reliable validated assay. These include matrix effects, biological significance and reliable tools for generating data.
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MiraiBio
Workshop Title: MasterPlex - The Next Generation From Acquisition to Analysis
Presenter: Allan Minn, MiraiBio Senior Software Engineer and Lead Designer of MasterPlex
The ultimate flexibility of the next generation MasterPlex software suite will be covered in this workshop. A demonstration of MasterPlex CT, a new data acquisition software platform, will provide highlights including automated startup and maintenance procedures, readymade kit configurations, wizard driven interface, and remote monitoring capabilities. The ease of integration between MasterPlex CT and MasterPlex QT, analysis application for quantitative assays, as well as MasterPlex GT, analysis software for genotyping assays, will also be covered.
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BMD
Workshop Title: How to Bring a Fabulous Concept to Routine Labs: From Luminex xMAP® Technology to the FIDIS™ System
Presenters: 'Industry Adaptation of xMAP® Technology to Routine Kits' - Karine Mignon-Godefroy, BMD R&D Lab Director
'High Throughput Modular, Flexible Automation - the CARIS™-FIDIS™ Workstation' - John Griffiths, VP Sales and Marketing, Scimedx Corp.
BMD's expertise is focused on the development and manufacturing of autoimmune and infectious diseases tests, Elisa and Immunodots. In April 2003, four Luminex based (FIDIS™) kits were launched in Europe. The FDA cleared the first FIDIS kit in September 2004. BMD rapidly acknowledged the need for automation and standardization of the technology, and to replace a well established existing method in a routine lab the offer must be superior. Superiority means increased safety, saved time to result, saved cost and increased efficiency of the treatment for the patient, by adding more valuable information. Today the CARIS™-FIDIS™ modular-flexible workstation and the associated kits developed by BMD, offers routine labs a choice that is by many aspects superior to traditional random access analyzers or Elisa processors.
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BioSource
Workshop Title: Sample Handling and Preparation for Multiplex Analysis
Presenter: Dr. Hans T. Beernink, BioSource Director of Immunology Assay Research and Development.
The power and versatility of multiplexed immunoassays greatly facilitates analysis in a wide range of biologically diverse sample types. However, many of these samples necessitate special consideration due to their composition, unique handling requirements, or sample-size limitations. Tissues or cultured-cell lysates, for example, require processing prior to assay and the methods chosen for this processing can dramatically influence the result. Likewise, samples such as cerebrospinal, synovial, or lavage fluids present special considerations for analysis due, in part, to their composition and collection. Using a case-study approach we describe several samples types and their associated problems, highlighting methods and proposed solutions.
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EraGen
Workshop Title: Introduction to MultiCode®-PLx Technology and New Applications for Nucleic Acid Based Diagnostics
Presenters: Scott Johnson, Ph.D. and Brad Pietz
This workshop will cover novel “base pair” chemistry and how it forms the foundation of the highly specific and sensitive MultiCode-PLx product line. MultiCode-PLx Systems are multiplexed, qualitative nucleic acid based systems that integrate with the Luminex instrument. The MultiCode-PLx system provides accurate results in less than three hours and is fully automatable.
An example of a clinical application will be demonstrated with data by The Blood Center of Southeastern Wisconsin.
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Genospectra
Workshop Title: QuantiGene™ Plex Reagent System: Multiplexed Gene Expression Analysis Directly from Cell Lysate and Tissue Homogenate
Presenter: Michelle Lyles, Ph.D., Genospectra Vice President, Marketing and Sales
The QuantiGene™ Plex Reagent System, powered by branched DNA (bDNA) and Luminex xMAP technology, enables precise and simultaneous quantification of up to 40 mRNA targets directly from cell lysates and tissue homogenates without the need for RNA purification, reverse transcription, or amplification. QuantiGene Plex is an extension of QuantiGene, the plate-based bDNA method. In both platforms, specificity is driven through cooperative hybridization between target-specific probes and universal capture probes and sensitivity is driven by bDNA-mediated signal amplification. QuantiGene Plex is sensitive and reproducible, capable of detecting single transcript of mRNA from as few as 25,000 cells and with intra-plate and inter-plate CVs of less than 10% and 15%, respectively. To demonstrate the utility of QuantiGene Plex, three 10-plex panels were developed to quantify expression changes of genes involved in the proinflammatory and apoptosis stimulation pathways. The results demonstrate that QuantiGene Plex provides a powerful means to quantify changes ingene expression from a multiple target genes in large sample populations.
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Ambion Diagnostics
Workshop Title: Selection, Validation & Implementation of a Bead-Array for Complex Genetic Testing
Presenter: Brian K. DuChateau, Ph.D., D(ABMLI), Director Product Development, Diagnostic Laboratories, The Blood Center of Southeastern Wisconsin
With the rapid introduction of new technologies in genetic testing, laboratories are faced with increased challenges in platform selection, assay development, validation and implementation. This workshop will explore the clinical application of liquid bead array technology, and important considerations for selecting a complex genetic test.
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