Support Resources

Support resources to help users make the most of their Luminex systems.

Protein, Nucleic Acid and xTAG® Protocols

Protein Protocols

DocumentLanguageTypeDownload
Protein Buffers List Englishpdf pdfImgdownload
Protein Coupling Protocol for MicroPlex® MicrospheresEnglishpdf pdfImgdownload
Protein Coupling Protocol for MagPlex® MicrospheresEnglishpdf pdfImgdownload
Confirmation of Antibody Coupling ProtocolEnglishpdf pdfImgdownload
LumAvidin® Binding Protocol Englishpdf pdfImgdownload
Capture Sandwich Immunoassay Protocols   
Recommended Protocol for Capture Sandwich Immunoassay Using MicroPlex Microspheres - Washed ProtocolEnglishpdf pdfImgdownload
Recommended Protocol for Capture Sandwich Immunoassay Using MicroPlex Microspheres – No Wash ProtocolEnglishpdf pdfImgdownload
Recommended Protocol for Capture Sandwich Immunoassay Using MagPlex Microspheres – Washed ProtocolEnglishpdf pdfImgdownload
Serological Immunoassay Protocols   
Recommended Protocol for Indirect (Antibody Capture)/Serological Immunoassay Using MicroPlex Microspheres – Washed ProtocolEnglishpdf pdfImgdownload
Recommended Protocol for Indirect (Antibody Capture)/Serological Immunoassay Using MagPlex Microspheres – Washed ProtocolEnglishpdf pdfImgdownload
Competitive Immunoassay Protocols    
Recommended Protocol for a Competitive Immunoassay using Antibody-Coupled Microspheres Englishpdf pdfImgdownload
Recommended Protocol for a Competitive Immunoassay using Antigen-Coupled Microspheres Englishpdf pdfImgdownload
Combined Capture Sandwich/Competitive ImmunoassayEnglishpdf pdfImgdownload

 

Nucleic Acid Protocols - Direct Hybridization

DocumentLanguageTypeDownload
DNA Buffer ListEnglishpdf pdfImgdownload
Recommendations for Probe/Primer Design for Direct Hybridization of Nucleic Acids Englishpdf pdfImgdownload
Sample Protocol for Coupling Oligonucleotides to MicroPlex MicrospheresEnglishpdf pdfImgdownload
Sample Protocol for Coupling Oligonucleotides to MagPlex Microspheres Englishpdf pdfImgdownload
Sample Protocol for Oligonucleotide HybridizationEnglishpdf pdfImgdownload
Sample Protocol for Direct DNA Hybridization to MicroPlex Microspheres - Washed Protocol Englishpdf pdfImgdownload
Sample Protocol for Direct DNA Hybridization to MagPlex Microspheres - Washed ProtocolEnglishpdf pdfImgdownload

 

Nucleic Acid Protocols - xTAG Technology

DocumentLanguageTypeDownload
xTAG Buffer ListEnglishpdf pdfImgdownload
Sample Protocol for Hybridization to MagPlex-TAG Microspheres - Washed ProtocolEnglishpdf pdfImgdownload
Sample Protocol for Hybridization to MagPlex-TAG Microspheres - No Wash Protocol Englishpdf pdfImgdownload
Sample Protocol for Hybridization of Biotin-TAG Oligonucleotides to MagPlex-TAG Microspheres Englishpdf pdfImgdownload
MagPlex-TAG Microspheres OverviewEnglishpdf pdfImgdownload
Custom Nucleic Acid Detection Assay ProtocolsEnglishpdf pdfImgdownload
Allele-Specific Primer Extension (ASPE)   
Recommendations for xTAG Allele-Specific Primer Extension (ASPE) Primer Design Englishpdf pdfImgdownload
Sample Protocol for Allele-Specific Primer Extension (ASPE) and Hybridization to MagPlex-TAG Microspheres - Washed Protocol Englishpdf pdfImgdownload
Sample Protocol for Allele-Specific Primer Extension (ASPE) and Hybridization to MagPlex-TAG Microspheres - No Wash ProtocolEnglishpdf pdfImgdownload
Oligonucleotide Ligation Assay (OLA)   
Recommendations for xTAG Oligonucleotide Ligation Assay (OLA) Probe Design Englishpdf pdfImgdownload
Sample Protocol for Oligonucleotide Ligation Assay (OLA) and Hybridization to MagPlex-TAG Microspheres - Washed ProtocolEnglishpdf pdfImgdownload
Sample Protocol for Oligonucleotide Ligation Assay (OLA) and Hybridization to MagPlex-TAG Microspheres - No Wash ProtocolEnglishpdf pdfImgdownload

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Target Values

Luminex 100/200 Calibration and Verification Kits for xPONENT 3.1
The lot specific information below is contained in the CD provided with each Calibration and Verification Kit. The target values can also be downloaded and saved to your Luminex 100/200 system. The Certificates of Quality are available below as well.

Calibration Kit LX200-CAL-K25; Lot Number Performance Verification Kit LX200-CON-K25; Lot Number
Target Value File (lxl)Certificate of Quality Target Value File (lxl)Certificate of Quality
B20798B20798   
B21327B21327 B20568B20568
B22989B22989 B21452B21452
B23319B23319 B23149B23149
B24774B24774 B24053B24053
B25346B25346 B24737B24737
B25629B25629 B25213B25213
B26209B26209 B26330B25947
B26965B26965  B26330
B27341B27341 B27063B27063
B27850B27850 B27565B27565
B28362B28362 B27972B27972
   B28250B28250

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Calibration Microspheres
Target Values are detailed in the following Certificate of Quality documents listed by lot number.

L100-CAL1 Lot NumberL100-CAL2 Lot NumberMCAL1 Lot Number
  B17083
B17265B17266B17415
B17522B17523B20427
B18628B18629B22610
B19650B20429 B24401
B20426B20601B25639
B20600 B20676B26964
B21094B21338B27083
B21521B21535 
B21534B21667  
B22274B22479 
B22923B23158 
B23385B24086 
B24166B25354 
B25090B25458 
B25615B25836 
B25948B26215 
B26156B26517 
B26440B26966 
B27148B27451 
B27450B27750 
B27945  
B28124  

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Control Microspheres 
Target Values are detailed in the following Certificate of Quality documents listed by lot number. 

L100-CON1 Lot NumberL100-CON2 Lot NumberMCON1 Lot Number
  B17416
  B18564
B17459B17526B21214
B17524B19606 B23431
B19927B20501B24667
B20770B21339B26167
B21565B21989B27064
B22275B23654B27084
B22456B22997 
B23753B24736 
B24726B25446 
B24988B25999 
B24858B27150 
B26441B27453 
B27149B26550 
B27452B28078 
B28077
  

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FLEXMAP 3D Calibration and Verification Kits
The lot specific information below is contained in the CD provided with each Calibration and Verification Kit. The target values can also be downloaded and saved to your FLEXMAP 3D system. The Certificates of Quality are available below as well.

Calibration Kit F3D-CAL-K25; Lot Number Performance Verification Kit F3D-PVER-K25; Lot Number
Target Value File (lxl)Certificate of Quality Target Value File (lxl)Certificate of Quality
 B18622 B17460 B19934 B19934
B21519B18622 B22400B22400
B23320B21519 B22981B22981
B26384B23320 B23121B23121
B28575
B24772 B25381B25381
 B24773 B27646B27646
 B26384 B28510B28510
 B28575   

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MAGPIX Calibration and Verifcation Kits
The lot specific information below is contained in the CD provided with each Calibration and Verification Kit. The target values can also be downloaded and saved to your MAGPIX system. The Certificates of Quality are available below as well.

Calibration Kit MPX-CAL-K25; Lot Number Performance Verification Kit MPX-PVER-K25; Lot Number
Target Value File (lxl)Certificate of Quality Target Value File (lxl)Certificate of Quality
B22576B22576 B22617B22617
B24327B24327 B24054B24054
B24795B24795 B24506B24506
B25076B25076 B24452B24452
B25077 B25077  B24935B24935
B25078 B25078  B25343B25343
B26418B26418 B25911B25911
B27801B27801 B26124B26124
B28293B28293 B27097B27097
B28313B28313 B27621B27621
   B27955B27955
   B27939B27939
   B28305B28305
   B28332B28332

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Recommended Materials

These Recommended Materials are intended for non-IVD (in vitro diagnostic) use. Included are items evaluated by Luminex and found to be compatible with xMAP® Technology.  Specific items may or may not be necessary, depending on application and assay format.

DocumentLanguageTypeDownload
Recommended Materials for Magnetic MicrospheresEnglishpdf pdfImgdownload
Reagent ListEnglishpdf pdfImgdownload
Recommended ConsumablesEnglishpdf pdfImgdownload
Recommended EquipmentEnglishpdf pdfImgdownload
Source ListEnglishpdf pdfImgdownload

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