Support Resources for Researchers

Protein, Nucleic Acid and xTAG^® Protocols

Protein Protocols

Document	Language	Type	Download
Protein Buffers List	English	pdf
Protein Coupling Protocol for MicroPlex® Microspheres	English	pdf
Protein Coupling Protocol for MagPlex® Microspheres	English	pdf
Confirmation of Antibody Coupling Protocol	English	pdf
LumAvidin® Binding Protocol	English	pdf
Capture Sandwich Immunoassay Protocols
Recommended Protocol for Capture Sandwich Immunoassay Using MicroPlex Microspheres - Washed Protocol	English	pdf
Recommended Protocol for Capture Sandwich Immunoassay Using MicroPlex Microspheres – No Wash Protocol	English	pdf
Recommended Protocol for Capture Sandwich Immunoassay Using MagPlex Microspheres – Washed Protocol	English	pdf
Serological Immunoassay Protocols
Recommended Protocol for Indirect (Antibody Capture)/Serological Immunoassay Using MicroPlex Microspheres – Washed Protocol	English	pdf
Recommended Protocol for Indirect (Antibody Capture)/Serological Immunoassay Using MagPlex Microspheres – Washed Protocol	English	pdf
Competitive Immunoassay Protocols
Recommended Protocol for a Competitive Immunoassay using Antibody-Coupled Microspheres	English	pdf
Recommended Protocol for a Competitive Immunoassay using Antigen-Coupled Microspheres	English	pdf
Combined Capture Sandwich/Competitive Immunoassay	English	pdf

Nucleic Acid Protocols - Direct Hybridization

Document	Language	Type	Download
DNA Buffer List	English	pdf
Recommendations for Probe/Primer Design for Direct Hybridization of Nucleic Acids	English	pdf
Sample Protocol for Coupling Oligonucleotides to MicroPlex Microspheres	English	pdf
Sample Protocol for Coupling Oligonucleotides to MagPlex Microspheres	English	pdf
Sample Protocol for Oligonucleotide Hybridization	English	pdf
Sample Protocol for Direct DNA Hybridization to MicroPlex Microspheres - Washed Protocol	English	pdf
Sample Protocol for Direct DNA Hybridization to MagPlex Microspheres - Washed Protocol	English	pdf

Nucleic Acid Protocols - xTAG Technology

Document	Language	Type	Download
xTAG Buffer List	English	pdf
Sample Protocol for Hybridization to MagPlex-TAG Microspheres - Washed Protocol	English	pdf
Sample Protocol for Hybridization to MagPlex-TAG Microspheres - No Wash Protocol	English	pdf
Sample Protocol for Hybridization of Biotin-TAG Oligonucleotides to MagPlex-TAG Microspheres	English	pdf
MagPlex-TAG Microspheres Overview	English	pdf
Custom Nucleic Acid Detection Assay Protocols	English	pdf
Allele-Specific Primer Extension (ASPE)
Recommendations for xTAG Allele-Specific Primer Extension (ASPE) Primer Design	English	pdf
Sample Protocol for Allele-Specific Primer Extension (ASPE) and Hybridization to MagPlex-TAG Microspheres - Washed Protocol	English	pdf
Sample Protocol for Allele-Specific Primer Extension (ASPE) and Hybridization to MagPlex-TAG Microspheres - No Wash Protocol	English	pdf
Oligonucleotide Ligation Assay (OLA)
Recommendations for xTAG Oligonucleotide Ligation Assay (OLA) Probe Design	English	pdf
Sample Protocol for Oligonucleotide Ligation Assay (OLA) and Hybridization to MagPlex-TAG Microspheres - Washed Protocol	English	pdf
Sample Protocol for Oligonucleotide Ligation Assay (OLA) and Hybridization to MagPlex-TAG Microspheres - No Wash Protocol	English	pdf

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Technology Tips

Document	Language	Type	Download
Antibody Post-Coupling Stability	English	pdf
Antibody Coupling Results	English	pdf
Comparison of Streptavidin-Phycoerythrin Conjugates	English	pdf
Effect of Mismatch Position and Type on SNP Genotyping	English	pdf
Effects of EDC on Oligonucleotide Coupling Efficiency	English	pdf
High Background in Serology Assays	English	pdf
Hybridization Time of PCR Amplified Targets	English	pdf
Incompatible Buffers and Solvents	English	pdf
MicroPlex Technology to Detect mRNA	English	pdf
Oligo-Coupled Microsphere Stability	English	pdf
Oligo-Coupled Microsphere Storage Buffer	English	pdf
Oligo Hybridization Results	English	pdf
PCR Hybridization Results	English	pdf
Recommendations for Scaling Up or Scaling Down Antibody Coupling Reactions	English	pdf
Recommendations for Scaling Up or Scaling Down Oligonucleotide Coupling Reactions	English	pdf
Relative Reporter Intensities	English	pdf
Stop Solution or Fixative to Stop a Reaction	English	pdf

Target Values

• Luminex^® 100/200™ Calibration and Verification Kits for xPONENT 3.1
• Calibration Microspheres
• Control Microspheres
• FLEXMAP^® 3D Calibration and Verification Kits
• MAGPIX^® Calibration and Verification Kits

Luminex 100/200 Calibration and Verification Kits for xPONENT 3.1
The lot specific information below is contained in the CD provided with each Calibration and Verification Kit. The target values can also be downloaded and saved to your Luminex 100/200 system. The Certificates of Quality are available below as well.

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Calibration Microspheres
Target Values are detailed in the following Certificate of Quality documents listed by lot number.

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Control Microspheres 
Target Values are detailed in the following Certificate of Quality documents listed by lot number. 

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FLEXMAP 3D Calibration and Verification Kits
The lot specific information below is contained in the CD provided with each Calibration and Verification Kit. The target values can also be downloaded and saved to your FLEXMAP 3D system. The Certificates of Quality are available below as well.

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MAGPIX Calibration and Verifcation Kits
The lot specific information below is contained in the CD provided with each Calibration and Verification Kit. The target values can also be downloaded and saved to your MAGPIX system. The Certificates of Quality are available below as well.

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Recommended Materials

These Recommended Materials are intended for non-IVD (in vitro diagnostic) use. Included are items evaluated by Luminex and found to be compatible with xMAP^® Technology.  Specific items may or may not be necessary, depending on application and assay format.

Document	Language	Type	Download
Recommended Materials for Magnetic Microspheres	English	pdf
Reagent List	English	pdf
Recommended Consumables	English	pdf
Recommended Equipment	English	pdf
Source List	English	pdf