Support Resources for Researchers

Support resources to help users make the most of their Luminex systems.

Protein, Nucleic Acid and xTAG® Protocols

Protein Protocols

DocumentLanguageTypeDownload
Protein Buffers List Englishpdf pdfImgdownload
Protein Coupling Protocol for MicroPlex® MicrospheresEnglishpdf pdfImgdownload
Protein Coupling Protocol for MagPlex® MicrospheresEnglishpdf pdfImgdownload
Confirmation of Antibody Coupling ProtocolEnglishpdf pdfImgdownload
LumAvidin® Binding Protocol Englishpdf pdfImgdownload
Capture Sandwich Immunoassay Protocols   
Recommended Protocol for Capture Sandwich Immunoassay Using MicroPlex Microspheres - Washed ProtocolEnglishpdf pdfImgdownload
Recommended Protocol for Capture Sandwich Immunoassay Using MicroPlex Microspheres – No Wash ProtocolEnglishpdf pdfImgdownload
Recommended Protocol for Capture Sandwich Immunoassay Using MagPlex Microspheres – Washed ProtocolEnglishpdf pdfImgdownload
Serological Immunoassay Protocols   
Recommended Protocol for Indirect (Antibody Capture)/Serological Immunoassay Using MicroPlex Microspheres – Washed ProtocolEnglishpdf pdfImgdownload
Recommended Protocol for Indirect (Antibody Capture)/Serological Immunoassay Using MagPlex Microspheres – Washed ProtocolEnglishpdf pdfImgdownload
Competitive Immunoassay Protocols    
Recommended Protocol for a Competitive Immunoassay using Antibody-Coupled Microspheres Englishpdf pdfImgdownload
Recommended Protocol for a Competitive Immunoassay using Antigen-Coupled Microspheres Englishpdf pdfImgdownload
Combined Capture Sandwich/Competitive ImmunoassayEnglishpdf pdfImgdownload

 

Nucleic Acid Protocols - Direct Hybridization

DocumentLanguageTypeDownload
DNA Buffer ListEnglishpdf pdfImgdownload
Recommendations for Probe/Primer Design for Direct Hybridization of Nucleic Acids Englishpdf pdfImgdownload
Sample Protocol for Coupling Oligonucleotides to MicroPlex MicrospheresEnglishpdf pdfImgdownload
Sample Protocol for Coupling Oligonucleotides to MagPlex Microspheres Englishpdf pdfImgdownload
Sample Protocol for Oligonucleotide HybridizationEnglishpdf pdfImgdownload
Sample Protocol for Direct DNA Hybridization to MicroPlex Microspheres - Washed Protocol Englishpdf pdfImgdownload
Sample Protocol for Direct DNA Hybridization to MagPlex Microspheres - Washed ProtocolEnglishpdf pdfImgdownload

 

Nucleic Acid Protocols - xTAG Technology

DocumentLanguageTypeDownload
xTAG Buffer ListEnglishpdf pdfImgdownload
Sample Protocol for Hybridization to MagPlex-TAG Microspheres - Washed ProtocolEnglishpdf pdfImgdownload
Sample Protocol for Hybridization to MagPlex-TAG Microspheres - No Wash Protocol Englishpdf pdfImgdownload
Sample Protocol for Hybridization of Biotin-TAG Oligonucleotides to MagPlex-TAG Microspheres Englishpdf pdfImgdownload
MagPlex-TAG Microspheres OverviewEnglishpdf pdfImgdownload
Custom Nucleic Acid Detection Assay ProtocolsEnglishpdf pdfImgdownload
Allele-Specific Primer Extension (ASPE)   
Recommendations for xTAG Allele-Specific Primer Extension (ASPE) Primer Design Englishpdf pdfImgdownload
Sample Protocol for Allele-Specific Primer Extension (ASPE) and Hybridization to MagPlex-TAG Microspheres - Washed Protocol Englishpdf pdfImgdownload
Sample Protocol for Allele-Specific Primer Extension (ASPE) and Hybridization to MagPlex-TAG Microspheres - No Wash ProtocolEnglishpdf pdfImgdownload
Oligonucleotide Ligation Assay (OLA)   
Recommendations for xTAG Oligonucleotide Ligation Assay (OLA) Probe Design Englishpdf pdfImgdownload
Sample Protocol for Oligonucleotide Ligation Assay (OLA) and Hybridization to MagPlex-TAG Microspheres - Washed ProtocolEnglishpdf pdfImgdownload
Sample Protocol for Oligonucleotide Ligation Assay (OLA) and Hybridization to MagPlex-TAG Microspheres - No Wash ProtocolEnglishpdf pdfImgdownload

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Target Values

Luminex 100/200 Calibration and Verification Kits for xPONENT 3.1
The lot specific information below is contained in the CD provided with each Calibration and Verification Kit. The target values can also be downloaded and saved to your Luminex 100/200 system. The Certificates of Quality are available below as well.

Calibration Kit LX200-CAL-K25; Lot Number   Performance Verification Kit LX200-CON-K25; Lot Number
Target Value File (lxl) Certificate of Quality   Target Value File (lxl) Certificate of Quality
B30124 B30124   B30520 B30520
B30238 B30238   B30739 B30739
B30981 B30981   B30859 B30859
B31539 B31539   B31122 B31122
B32256 B32256   B31985 B31985
B32885 B32885   B32850 B32850
B33324 B33324   B32886 B32886
B33909 B33909   B33743 B33743
B34736 B34736   B33910 B33910
B35259 B35259   B34517 B34517
B36301 B36301   B34737 B34737
B37129 B37129   B35221 B35221
B37370 B37370   B35688 B35688
B37803 B37803   B36302 B36302
B38292 B38292   B36982 B36982
B38761 B38761   B37167 B37167
B40034 B40034   B37426 B37426
B41012 B41012   B37802 B37802
B41484 B41484   B38293 B38293
      B39060 B39060
      B40744 B40744
      B41013 B41013

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Calibration Microspheres
Target Values are detailed in the following Certificate of Quality documents listed by lot number.

L100-CAL1 Lot Number L100-CAL2 Lot Number MCAL1 Lot Number
B29562 B29561 B29522
B29568 B29569 B29572
B30229 B30018 B32361
B30869 B30870 B32887
B31314 B31315 B33913
B31543 B31544 B35520
B32259 B32260 B36312
B32944 B32945 B37372
B33156 B33157 B38719
B33282 B33283  
B34428 B34429  
B35239 B35240  
B35684 B35729  
B35728 B36168  
B36167 B36821  
B36353 B37188  
B37116 B37371  
B37187 B38765  
B38104 B39594  
B39593 B40643  
B40642 B41029  
B41028    
B41109    

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Control Microspheres 
Target Values are detailed in the following Certificate of Quality documents listed by lot number. 

L100-CON1 Lot Number L100-CON2 Lot Number MCON1 Lot Number
B29499 B29442 B29515
B29570 B29571 B29573
B30409 B30410 B30939
B30983 B30982 B30959
B31670 B31671 B33212
B32469 B32470 B33764
B32946 B32947 B34941
B33941 B33940 B35816
B34430 B34431 B36352
B36290 B36077 B38357
B36965 B36291 B40910
B37451 B36964  
B38762 B37452  
B40644 B38763  
  B40645  

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FLEXMAP 3D Calibration and Verification Kits
The lot specific information below is contained in the CD provided with each Calibration and Verification Kit. The target values can also be downloaded and saved to your FLEXMAP 3D system. The Certificates of Quality are available below as well.

Calibration Kit F3D-CAL-K25; Lot Number   Performance Verification Kit F3D-PVER-K25; Lot Number
Target Value File (lxl) Certificate of Quality   Target Value File (lxl) Certificate of Quality
B33750 B33750   B33865 B33865
B35628 B35628   B39208 B39208
B35988 B35988   B39209 B39209
B36573 B36573   B39638 B39638
B39165 B39165   B41707 B41707
B42038 B42038      

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MAGPIX Calibration and Verifcation Kits
The lot specific information below is contained in the CD provided with each Calibration and Verification Kit. The target values can also be downloaded and saved to your MAGPIX system. The Certificates of Quality are available below as well.

Calibration Kit MPX-CAL-K25; Lot Number   Performance Verification Kit MPX-PVER-K25; Lot Number
Target Value File (lxl) Certificate of Quality   Target Value File (lxl) Certificate of Quality
B30228 B30228   B29972 B29972
B30740 B30740   B30013 B30013
B30934 B30934   B30823 B30823
B32536 B32536   B30935 B30935
B32702 B32702   B32454 B32454
B32847 B32847   B32926 B32926
B35220 B35220   B33098 B33098
B35550 B35550   B34505 B34505
B36348 B36348   B35519 B35519
B37475 B37475   B35986 B35986
B38155 B38155   B36060 B36060
B38670 B38670   B36330 B36330
B39584 B39584   B37160 B37160
B40913 B40913   B37455 B37455
B40975 B40975   B37690 B37690
      B37809 B37809
      B38544 B38544
      B39085 B39085
      B39570 B39570
      B40035 B40035
      B41014 B41014

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Recommended Materials

These Recommended Materials are intended for non-IVD (in vitro diagnostic) use. Included are items evaluated by Luminex and found to be compatible with xMAP® Technology.  Specific items may or may not be necessary, depending on application and assay format.

Document Language Type Download
Recommended Materials for Magnetic Microspheres English pdf pdfImg download
Reagent List English pdf pdfImg download
Recommended Consumables English pdf pdfImg download
Recommended Equipment English pdf pdfImg download
Source List English pdf pdfImg download