Support Resources for Researchers

Support resources to help users make the most of their Luminex systems.

Protein, Nucleic Acid and xTAG® Protocols

Protein Protocols

DocumentLanguageTypeDownload
Protein Buffers List Englishpdf pdfImgdownload
Protein Coupling Protocol for MicroPlex® MicrospheresEnglishpdf pdfImgdownload
Protein Coupling Protocol for MagPlex® MicrospheresEnglishpdf pdfImgdownload
Confirmation of Antibody Coupling ProtocolEnglishpdf pdfImgdownload
LumAvidin® Binding Protocol Englishpdf pdfImgdownload
Capture Sandwich Immunoassay Protocols   
Recommended Protocol for Capture Sandwich Immunoassay Using MicroPlex Microspheres - Washed ProtocolEnglishpdf pdfImgdownload
Recommended Protocol for Capture Sandwich Immunoassay Using MicroPlex Microspheres – No Wash ProtocolEnglishpdf pdfImgdownload
Recommended Protocol for Capture Sandwich Immunoassay Using MagPlex Microspheres – Washed ProtocolEnglishpdf pdfImgdownload
Serological Immunoassay Protocols   
Recommended Protocol for Indirect (Antibody Capture)/Serological Immunoassay Using MicroPlex Microspheres – Washed ProtocolEnglishpdf pdfImgdownload
Recommended Protocol for Indirect (Antibody Capture)/Serological Immunoassay Using MagPlex Microspheres – Washed ProtocolEnglishpdf pdfImgdownload
Competitive Immunoassay Protocols    
Recommended Protocol for a Competitive Immunoassay using Antibody-Coupled Microspheres Englishpdf pdfImgdownload
Recommended Protocol for a Competitive Immunoassay using Antigen-Coupled Microspheres Englishpdf pdfImgdownload
Combined Capture Sandwich/Competitive ImmunoassayEnglishpdf pdfImgdownload

 

Nucleic Acid Protocols - Direct Hybridization

DocumentLanguageTypeDownload
DNA Buffer ListEnglishpdf pdfImgdownload
Recommendations for Probe/Primer Design for Direct Hybridization of Nucleic Acids Englishpdf pdfImgdownload
Sample Protocol for Coupling Oligonucleotides to MicroPlex MicrospheresEnglishpdf pdfImgdownload
Sample Protocol for Coupling Oligonucleotides to MagPlex Microspheres Englishpdf pdfImgdownload
Sample Protocol for Oligonucleotide HybridizationEnglishpdf pdfImgdownload
Sample Protocol for Direct DNA Hybridization to MicroPlex Microspheres - Washed Protocol Englishpdf pdfImgdownload
Sample Protocol for Direct DNA Hybridization to MagPlex Microspheres - Washed ProtocolEnglishpdf pdfImgdownload

 

Nucleic Acid Protocols - xTAG Technology

DocumentLanguageTypeDownload
xTAG Buffer ListEnglishpdf pdfImgdownload
Sample Protocol for Hybridization to MagPlex-TAG Microspheres - Washed ProtocolEnglishpdf pdfImgdownload
Sample Protocol for Hybridization to MagPlex-TAG Microspheres - No Wash Protocol Englishpdf pdfImgdownload
Sample Protocol for Hybridization of Biotin-TAG Oligonucleotides to MagPlex-TAG Microspheres Englishpdf pdfImgdownload
MagPlex-TAG Microspheres OverviewEnglishpdf pdfImgdownload
Custom Nucleic Acid Detection Assay ProtocolsEnglishpdf pdfImgdownload
Allele-Specific Primer Extension (ASPE)   
Recommendations for xTAG Allele-Specific Primer Extension (ASPE) Primer Design Englishpdf pdfImgdownload
Sample Protocol for Allele-Specific Primer Extension (ASPE) and Hybridization to MagPlex-TAG Microspheres - Washed Protocol Englishpdf pdfImgdownload
Sample Protocol for Allele-Specific Primer Extension (ASPE) and Hybridization to MagPlex-TAG Microspheres - No Wash ProtocolEnglishpdf pdfImgdownload
Oligonucleotide Ligation Assay (OLA)   
Recommendations for xTAG Oligonucleotide Ligation Assay (OLA) Probe Design Englishpdf pdfImgdownload
Sample Protocol for Oligonucleotide Ligation Assay (OLA) and Hybridization to MagPlex-TAG Microspheres - Washed ProtocolEnglishpdf pdfImgdownload
Sample Protocol for Oligonucleotide Ligation Assay (OLA) and Hybridization to MagPlex-TAG Microspheres - No Wash ProtocolEnglishpdf pdfImgdownload

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Target Values

Luminex 100/200 Calibration and Verification Kits for xPONENT 3.1
The lot specific information below is contained in the CD provided with each Calibration and Verification Kit. The target values can also be downloaded and saved to your Luminex 100/200 system. The Certificates of Quality are available below as well.

Calibration Kit LX200-CAL-K25; Lot Number   Performance Verification Kit LX200-CON-K25; Lot Number
Target Value File (lxl) Certificate of Quality   Target Value File (lxl) Certificate of Quality
B29291 B29291   B30520 B30520
B29292 B29292   B30739 B30739
B30124 B30124   B30859 B30859
B30238 B30238   B31122 B31122
B30981 B30981   B31985 B31985
B31539 B31539   B32850 B32850
B32256 B32256   B32886 B32886
B32885 B32885   B33743 B33743
B33324 B33324   B33910 B33910
B33909 B33909   B34517 B34517
B34736 B34736   B34737 B34737
B35259 B35259   B35221 B35221
B36301 B36301   B35688 B35688
B37129 B37129   B36302 B36302
B37370 B37370   B36982 B36982
B37803 B37803   B37167 B37167
B38292 B38292   B37426 B37426
B38761 B38761   B37802 B37802
      B38293 B38293
      B39060 B39060

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Calibration Microspheres
Target Values are detailed in the following Certificate of Quality documents listed by lot number.

L100-CAL1 Lot Number L100-CAL2 Lot Number MCAL1 Lot Number
B28124 B28366 B28860
B28587 B29217 B29522
B29290 B29561 B29572
B29562 B29569 B32361
B29568 B30018 B32887
B30229 B30870 B33913
B30869 B31315 B35520
B31314 B31544 B36312
B31543 B32260 B37372
B32259 B32945 B38719
B32944 B33157  
B33156 B33283  
B33282 B34429  
B34428 B35240  
B35239 B35729  
B35684 B36168  
B35728 B36821  
B36167 B37188  
B36353 B37371  
B37116 B38765  
B37187 B39594  
B38104    
B39593    

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Control Microspheres 
Target Values are detailed in the following Certificate of Quality documents listed by lot number. 

L100-CON1 Lot Number L100-CON2 Lot Number MCON1 Lot Number
B28077 B28078 B28748
B28822 B28823 B29515
B29499 B29442 B29573
B29570 B29571 B30939
B30409 B30410 B30959
B30983 B30982 B33212
B31670 B31671 B33764
B32469 B32470 B34941
B32946 B32947 B35816
B33941 B33940 B36352
B34430 B34431 B38357
B36290 B36077  
B36965 B36291  
B37451 B36964  
B38762 B37452  
  B38763  

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FLEXMAP 3D Calibration and Verification Kits
The lot specific information below is contained in the CD provided with each Calibration and Verification Kit. The target values can also be downloaded and saved to your FLEXMAP 3D system. The Certificates of Quality are available below as well.

Calibration Kit F3D-CAL-K25; Lot Number   Performance Verification Kit F3D-PVER-K25; Lot Number
Target Value File (lxl) Certificate of Quality   Target Value File (lxl) Certificate of Quality
B31073 B31073   B31627 B31627
B33750 B33750   B33865 B33865
B35628 B35628   B39208 B39208
B35988 B35988   B39209 B39209
B36573 B36573   B39638 B39638
B39165 B39165      

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MAGPIX Calibration and Verifcation Kits
The lot specific information below is contained in the CD provided with each Calibration and Verification Kit. The target values can also be downloaded and saved to your MAGPIX system. The Certificates of Quality are available below as well.

Calibration Kit MPX-CAL-K25; Lot Number   Performance Verification Kit MPX-PVER-K25; Lot Number
Target Value File (lxl) Certificate of Quality   Target Value File (lxl) Certificate of Quality
B28818 B28818   B28611 B28611
B29345 B29345   B29056 B29056
B30012 B30012   B29972 B29972
B30227 B30227   B30013 B30013
B30228 B30228   B30823 B30823
B30740 B30740   B30935 B30935
B30934 B30934   B32454 B32454
B32536 B32536   B32926 B32926
B32702 B32702   B33098 B33098
B32847 B32847   B34505 B34505
B35220 B35220   B35519 B35519
B35550 B35550   B35986 B35986
B36348 B36348   B36060 B36060
B37475 B37475   B36330 B36330
B38155 B38155   B37160 B37160
B38670 B38670   B37455 B37455
B39584 B39584   B37690 B37690
      B37809 B37809
      B38544 B38544
      B39085 B39085
      B39570 B39570
      B40035 B40035

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Recommended Materials

These Recommended Materials are intended for non-IVD (in vitro diagnostic) use. Included are items evaluated by Luminex and found to be compatible with xMAP® Technology.  Specific items may or may not be necessary, depending on application and assay format.

Document Language Type Download
Recommended Materials for Magnetic Microspheres English pdf pdfImg download
Reagent List English pdf pdfImg download
Recommended Consumables English pdf pdfImg download
Recommended Equipment English pdf pdfImg download
Source List English pdf pdfImg download