MagPlex^® Microspheres

Similar to MicroPlex^® Microspheres, MagPlex Microspheres are carboxylated polystyrene micro-particles, or “beads,” that have been dyed into spectrally distinct sets, or “regions”; allowing them to be individually identified by an xMAP^® Instrument. These uniquely coded beads provide a user an addressable substrate on which to perform multiple bio-analytical reactions in a single well.

All microspheres are general use components for further manufacture of xMAP bioassays.

MagPlex Microspheres are available at a concentration of 2.5 million and 12.5 million beads/mL and can be ordered in 1mL and 4mL vial sizes. MagPlex Microspheres are also offered in a variety of custome volumes. Please see the MagPlex Custom Volume Ordering Guidelines or contact Luminex to inquire about custom volume options.

IMPORTANT: MagPlex microspheres require the use of xPONENT^® software.

Click For More Information

Overview
Specifications
Ordering
Downloads
Training
Support

Overview

MagPlex Image

There are several advantages to MagPlex Microspheres over traditional MicroPlex Microspheres.

Since MagPlex Microspheres are magnetic, this allows them to be separated from solution quickly and easily with the use of a magnetic separator; improving percent recovery during handling and wash steps, and facilitating assay automation.

Additionally, MagPlex Microspheres are available in 500 unique regions, enabling the development of assays which can measure up to 500 different analytes in the same well.

Another, advantage of these beads is their universal compatibility with all xMAP Instruments. MagPlex Microspheres open the door to Luminex’s latest instrument, MAGPIX^®. Designed as a simple and easy to use instrument, with a smaller foot print, MAGPIX is also the most economical multiplex instrument on the market.

Features

Carboxylated
Superparamagnetic
500 Regions available
Compatible with all xMAP Instruments
Multiple vial sizes available
Multiple concentrations available

Benefits

Same reliable coupling chemistry as MicroPlex
5x Increased multiplex capability
Improved washing efficiency
Ideal for automated applications
No need for expensive filter plates
Now available in low concentration, for small scale and assay development applications

Specifications

MagPlex Microspheres are provided in purified water containing <0.1% w/v ProClin^® as a preservative. Product specifications are assured up to the expiration date stated on the label with proper handling and storage. The expiration date is intended to be the latest date at which the material should be used in further manufacture of bioassays. Although continued conformance to specifications is expected subsequent to further manufacture of the microspheres, users must characterize and control their formulations to assure assay specific performance.

Properties	Specification
Microsphere Concentration (microspheres/mL)	11.0 x 10⁶ to 14.5 x 10⁶
Medium	<0.1% ProClin in Water
Median Microsphere Density (g/mL)	1.10 ± 0.06
Mode Microsphere Diameter (µm)	6.5 ± 0.2
RP1 background	≤ 100
Classification Efficiency	> 80%
Misclassification	≤ 2.0%
Doublet Discrimination Peak	9,000 – 14,000

Storage

Store product upright at 2-8ºC dark. Avoid freezing. Avoid exposure to heat >55ºC for durations greater than 24 hours.

For a complete list of specifications see the MagPlex Product Information Sheet and other product related documents in the Downloads section.

Ordering Information

Online Order Management
Email: [email protected]
Phone: 1-512-336-3550
Toll Free: 1-866-401-5450
Fax: 1-512-219-0544
Fax: 31-162-408330 (EMEA, India)

Reagent Shipping Policy
All MagPlex Microsphere orders, 1 & 4 ml volumes, will usually ship within 3 business days of order receipt. However, back ordered items may have longer than normal lead times. Group A and Group B regions have a guaranteed minimum shelf life of 6 month from the date of shipment and Group C regions have a guaranteed minimum shelf life of 3 month from the shipment date. Please see the MagPlex Instrument Compatibility Guidelines for more information on region grouping.

Part Numbers

To ensure maximum compatibility and availability, new assay developers should always begin with the Group A regions; moving into Group B then Group C regions only when necessary for higher-plex assays.
NOTE: Group C regions are only available in 1mL vial sizes.
NOTE: Group C regions <100 have MC12XXX catalog numbers.
NOTE: [XXX] = region # >100

Vial Sizes:
[-YY] = [-01] for 1mL;
[-04] for 4mL; or
[-ID] for 1mL Low Concentration
Please contact Luminex to inquire about custom vial sizes.

Region	Part Number	Instrument Compatibility	Region Group
12	MC10012-YY (Available in Low Concentration)	All xMAP Instruments	Group A
13	MC10013-YY (Available in Low Concentration)	All xMAP Instruments	Group A
14	MC10014-YY (Available in Low Concentration)	All xMAP Instruments	Group A
15	MC10015-YY (Available in Low Concentration)	All xMAP Instruments	Group A
18	MC10018-YY (Available in Low Concentration)	All xMAP Instruments	Group A
19	MC10019-YY (Available in Low Concentration)	All xMAP Instruments	Group A
20	MC10020-YY (Available in Low Concentration)	All xMAP Instruments	Group A
21	MC10021-YY (Available in Low Concentration)	All xMAP Instruments	Group A
22	MC10022-YY (Available in Low Concentration)	All xMAP Instruments	Group A
25	MC10025-YY (Available in Low Concentration)	All xMAP Instruments	Group A
26	MC10026-YY	All xMAP Instruments	Group A
27	MC10027-YY	All xMAP Instruments	Group A
28	MC10028-YY	All xMAP Instruments	Group A
29	MC10029-YY	All xMAP Instruments	Group A
30	MC10030-YY	All xMAP Instruments	Group A
33	MC10033-YY	All xMAP Instruments	Group A
34	MC10034-YY	All xMAP Instruments	Group A
35	MC10035-YY	All xMAP Instruments	Group A
36	MC10036-YY	All xMAP Instruments	Group A
37	MC10037-YY	All xMAP Instruments	Group A
38	MC10038-YY	All xMAP Instruments	Group A
39	MC10039-YY	All xMAP Instruments	Group A
42	MC10042-YY	All xMAP Instruments	Group A
43	MC10043-YY	All xMAP Instruments	Group A
44	MC10044-YY	All xMAP Instruments	Group A
45	MC10045-YY	All xMAP Instruments	Group A
46	MC10046-YY	All xMAP Instruments	Group A
47	MC10047-YY	All xMAP Instruments	Group A
48	MC10048-YY	All xMAP Instruments	Group A
51	MC10051-YY	All xMAP Instruments	Group A
52	MC10052-YY	All xMAP Instruments	Group A
53	MC10053-YY	All xMAP Instruments	Group A
54	MC10054-YY	All xMAP Instruments	Group A
55	MC10055-YY	All xMAP Instruments	Group A
56	MC10056-YY	All xMAP Instruments	Group A
57	MC10057-YY	All xMAP Instruments	Group A
61	MC10061-YY	All xMAP Instruments	Group A
62	MC10062-YY	All xMAP Instruments	Group A
63	MC10063-YY	All xMAP Instruments	Group A
64	MC10064-YY	All xMAP Instruments	Group A
65	MC10065-YY	All xMAP Instruments	Group A
66	MC10066-YY	All xMAP Instruments	Group A
67	MC10067-YY	All xMAP Instruments	Group A
72	MC10072-YY	All xMAP Instruments	Group A
73	MC10073-YY	All xMAP Instruments	Group A
74	MC10074-YY	All xMAP Instruments	Group A
75	MC10075-YY	All xMAP Instruments	Group A
76	MC10076-YY	All xMAP Instruments	Group A
77	MC10077-YY	All xMAP Instruments	Group A
78	MC10078-YY	All xMAP Instruments	Group A
7	MC10007-YY	Luminex 100/200 and FLEXMAP 3D	Group B
8	MC10008-YY	Luminex 100/200 and FLEXMAP 3D	Group B
9	MC10009-YY	Luminex 100/200 and FLEXMAP 3D	Group B
49	MC10049-YY	Luminex 100/200 and FLEXMAP 3D	Group B
58	MC10058-YY	Luminex 100/200 and FLEXMAP 3D	Group B
59	MC10059-YY	Luminex 100/200 and FLEXMAP 3D	Group B
68	MC10068-YY	Luminex 100/200 and FLEXMAP 3D	Group B
69	MC10069-YY	Luminex 100/200 and FLEXMAP 3D	Group B
70	MC10070-YY	Luminex 100/200 and FLEXMAP 3D	Group B
79	MC10079-YY	Luminex 100/200 and FLEXMAP 3D	Group B
80	MC10080-YY	Luminex 100/200 and FLEXMAP 3D	Group B
81	MC10081-YY	Luminex 100/200 and FLEXMAP 3D	Group B
82	MC10082-YY	Luminex 100/200 and FLEXMAP 3D	Group B
83	MC10083-YY	Luminex 100/200 and FLEXMAP 3D	Group B
84	MC10084-YY	Luminex 100/200 and FLEXMAP 3D	Group B
85	MC10085-YY	Luminex 100/200 and FLEXMAP 3D	Group B
86	MC10086-YY	Luminex 100/200 and FLEXMAP 3D	Group B
87	MC10087-YY	Luminex 100/200 and FLEXMAP 3D	Group B
88	MC10088-YY	Luminex 100/200 and FLEXMAP 3D	Group B
89	MC10089-YY	Luminex 100/200 and FLEXMAP 3D	Group B
90	MC10090-YY	Luminex 100/200 and FLEXMAP 3D	Group B
91	MC10091-YY	Luminex 100/200 and FLEXMAP 3D	Group B
93	MC10093-YY	Luminex 100/200 and FLEXMAP 3D	Group B
94	MC10094-YY	Luminex 100/200 and FLEXMAP 3D	Group B
95	MC10095-YY	Luminex 100/200 and FLEXMAP 3D	Group B
96	MC10096-YY	Luminex 100/200 and FLEXMAP 3D	Group B
97	MC10097-YY	Luminex 100/200 and FLEXMAP 3D	Group B
98	MC10098-YY	Luminex 100/200 and FLEXMAP 3D	Group B
99	MC10099-YY	Luminex 100/200 and FLEXMAP 3D	Group B
100	MC10100-YY	Luminex 100/200 and FLEXMAP 3D	Group B
1	MC12001-01	FLEXMAP 3D Only	Group C
2	MC12002-01	FLEXMAP 3D Only	Group C
3	MC12003-01	FLEXMAP 3D Only	Group C
4	MC12004-01	FLEXMAP 3D Only	Group C
5	MC12005-01	FLEXMAP 3D Only	Group C
6	MC12006-01	FLEXMAP 3D Only	Group C
10	MC12010-01	FLEXMAP 3D Only	Group C
11	MC12011-01	FLEXMAP 3D Only	Group C
16	MC12016-01	FLEXMAP 3D Only	Group C
17	MC12017-01	FLEXMAP 3D Only	Group C
23	MC12023-01	FLEXMAP 3D Only	Group C
24	MC12024-01	FLEXMAP 3D Only	Group C
31	MC12031-01	FLEXMAP 3D Only	Group C
32	MC12032-01	FLEXMAP 3D Only	Group C
40	MC12040-01	FLEXMAP 3D Only	Group C
41	MC12041-01	FLEXMAP 3D Only	Group C
50	MC12050-01	FLEXMAP 3D Only	Group C
60	MC12060-01	FLEXMAP 3D Only	Group C
71	MC12071-01	FLEXMAP 3D Only	Group C
92	MC12092-01	FLEXMAP 3D Only	Group C
101+	MC10XXX-01	FLEXMAP 3D Only	Group C

Downloads

File	Language	Type	Size
MSDS: MagPlex-C Microspheres (US/Canada)	English	pdf	31k
How to convert ELISA format assays to xMAP using MagPlex beads for MAGPIX	English	pdf	317k
xMAP Antibody Coupling Kit User Manual	English	pdf	696k
Custom Nucleic Acid Detection	English	pdf	389k
Transferring Immunoassays MicroPlex to MagPlex White Paper	English	pdf	2,470k
Overcoming the Cost and Performance Limitations of ELISA with xMAP Technology	English	pdf	632k
MagPlex Microspheres Product Information Sheet	English	pdf	220k
MagPlex-C Magnetic Microspheres Performance on Magnetic Separators	English	pdf	32k
Recommended Protocol for Indirect (Antibody Capture)/Serological Immunoassay Using MagPlex Microspheres – Washed Protocol	English	pdf	78k
Sample Protocol for Direct DNA Hybridization to MagPlex Microspheres - Washed Protocol	English	pdf	27k
Sample Protocol for Coupling Oligonucleotides to MagPlex Microspheres	English	pdf	29k
Recommended Protocol for Capture Sandwich Immunoassay Using MagPlex Microspheres – Washed Protocol	English	pdf	27k
Protein Coupling Protocol for MagPlex Microspheres	English	pdf	27k
Magnetic Microspheres Recommended Materials	English	pdf	22k
Recommended Consumables	English	pdf	31k
Source List Recommended Materials	English	pdf	75k
Recommended Equipment	English	pdf	19k
Reagent List Recommended Materials	English	pdf	78k
MagPlex Manual Wash Method White Paper	English	pdf	3,110k
MagPlex Tricks and Tips White Paper	English	pdf	4,265k
MAGPIX Protein Assays Overview	English	pdf	1,218k
MAGPIX Nucleic Acid Assays Overview	English	pdf	434k
Nucleic Acid Reagent Overview	English	pdf	520k
Protein Reagent Overview	English	pdf	1,234k
Reagents Overview	English	pdf	568k
MagPlex® Microspheres Provide Superior Performance to Non-magnetic Beads in Prostate Cancer Assays	English	pdf	341k

Assay Development Tools

Building your own custom assays on the Luminex platform is a simple, easy process with our assay development tools. Purchase microsphere reagents from Luminex, supply oligos or proteins to coat the microspheres and follow our simple protocols to allow multiplexing of almost any assay needed by your lab.

Training Courses

Course Title	Format	Duration
Fundamental Assay Techniques, Nucleic Acid	Classroom	2 days
Fundamental Assay Techniques, Protein and Nucleic Acid	Classroom	3 days

Support

Support Resources	Technical Support	Field Service
Protocols	Online Support Login	Certified Service Providers
Technical Tips	Email: [email protected]	Maintenance and Support
Target Values	Phone: 512-381-4397	Luminex Central Repair Facility
Recommended Materials	Toll Free: 1-877-785-2323
	International: + 800-2939-4959

MagPlex® Microspheres