FLEXMAP 3D®
Taking the pioneering xMAP® Technology to the next dimension, the FLEXMAP 3D system combines differentially dyed fluorescent microsphere sets with an innovative instrument design to allow multiplexing of up to 500 unique assays within a single sample, both rapidly and precisely.
FLEXMAP 3D Overview
Flexible Multiplexing Platform
Fast read time, broad dynamic range, compatibility with front-end automation and laboratory information systems, and the ability to measure up to 500 genes or proteins from a small sample volume make FLEXMAP 3D Luminex’s most advanced multiplexing platform.
Benefits of FLEXMAP 3D
- Fast - Higher Throughput
Dual sample fluidics paths and increased sample injection speed result in a faster sampling rate. The FLEXMAP 3D system can process 384 samples and generate up to 144,000 data points in less than one hour. - Flexible - High Plexing Capability
The FLEXMAP 3D is compatible with magnetic and non-magnetic microspheres and can discriminate up to 500 different microsphere sets. The platform offers a large menu of commercial kits and reagents to develop custom assays in a wide variety of applications including nucleic acid assays, immunoassays, receptor ligand assays and enzymatic assays. - Versatile - Automation/LIS Compatibility
xPONENT® Software controls the FLEXMAP 3D system and offers interfacing options for Laboratory Information Systems (LIS) and front-end automation platforms. The system accommodates 96 and 384-well plate formats for enhanced sample volume flexibility and increased throughput. - Reliable
The FLEXMAP 3D system has multiple features allowing walk away start-up, shutdown and maintenance operations, and greatly enhances usability for High Throughput Screening (HTS) applications.
The FLEXMAP 3D system is a class 1(I) laser product.
Product Specifications
| Performance | |
| Multiplexing Capability | Up to 500 individual analytes |
| Dynamic Range | > 4.5 logs |
| General | |
| Physical Dimensions | 58.4 cm W x 63.5 cm D x 54.7 cm H (23” W x 25.7” D x 18” ) |
| Weight | No more than 200 lbs (91 Kg) |
| Plate Heater Operating Range | 35 – 60ºC (95 - 131ºF) |
| System Warm Up* | 30 minutes |
| Fluidics | |
| Sheath Fluid Pressure | 8 – 13 psi |
| Sample Injection Rate | 2 μl/second |
| Sample Uptake Volume | 10 – 200 μl |
| Optics | |
| Classification Laser | 638 nm |
| Reporter Laser | 532 nm |
| Reporter Detector | 565nm – 585nm |
* The FLEXMAP 3D system is a class 1(I) laser product.
xPONENT® Software Solutions
Data Acquisition and Analysis for Luminex Instrumentation
xPONENT is a modular, flexible, software package for control of the MAGPIX®, Luminex® 100/200™ and FLEXMAP 3D. The software’s graphical user interface follows the typical assay workflow but can be customized to meet the needs of a specific laboratory.
Software Configurations
xPONENT software is available with three levels of accessibility.
Basic
The basic xPONENT software package allows the user to acquire samples, perform analysis, and run system maintenance routines and remote web monitoring. This package includes the ability to calculate allelic ratios to analyze and/or normalize genotyping data.
Secure
The secure xPONENT package includes all the basic functionality, plus allows an administrator to set up user access levels.
21 CFR Part 11
For laboratories working under the FDA’s 21 CFR Part 11 regulations, xPONENT offers multilevel user management, full audit trail, electronic records and electronic signatures.
In addition to the access levels listed above, there are two additional licensable modules that add specific functions:
Automation Connectivity
The automation module along with a device driver enables communication between the reader and a front end automation platform. Luminex device drivers are offered by many automation providers. For more information on available drivers please contact Luminex Technical Support.
LIS Connectivity
This module allows communication with an external Laboratory Information System (LIS) database by allowing the export of patient result data and the import of patient sample information in ASTM format. Connection to your LIS system may require the use of middleware. For more information on middleware solutions please contact Luminex Technical Support.
Ordering Information
| Luminex Direct | Luminex Partners |
|---|---|
| Luminex Online Order Management Email: [email protected] Phone: 1-512-336-3550 Toll Free: 1-866-401-5450 Fax: 1-512-219-0544 Fax: 31-162-408330 (EMEA, India) | Bio-Rad Laboratories EMD Millipore Corporation Life Technologies |
| Product Description | Luminex Part Number | List Price USD | Description |
|---|---|---|---|
| Instrument | |||
| FLEXMAP 3D | Order Information | Versatile multiplexing platform | |
| Reagents | |||
| FLEXMAP 3D Calibration Kit | Order Information | xMAP® Calibrators for MagPlex® and MicroPlex® Classification Channels and Calibrator for Reporter Channel | |
| FLEXMAP 3D Performance Verification Kit | Order Information | xMAP Verification Reagents to Verify Calibration of MagPlex and MicroPlex Classification Channels and Reporter Channel | |
| Sheath Fluid | 40-75680 | Order Information | Sheath Concentrate Pack |
| 40-50000 | Order Information | 20 L Sheath Fluid | |
| Accessories | |||
| Sample Probe Needle | CN-0221-01 | Call for price | Stainless steel sampling needle for use with the FLEXMAP 3D system |
| Sample Probe Height Adjustment Kit | CN-0263-01 | Call for price | Probe height adjustment tools for use with the FLEXMAP 3D system |
| FLEXMAP 3D Off-Plate Reagent Block | CN-0225-01 | Call for price | Calibration, Performance Verification and Maintenance plate for performance testing of the FLEXMAP 3D system |
| Heater Block, 96-Well Microtiter Plate | CN-0224-01 | Call for price | Anodized aluminum heating block compatible with 96-well V-bottom plates for use on the FLEXMAP 3D system |
| Heater Block, 384-Well Microtiter Plate | CN-0223-01 | Call for price | Anodized aluminum heating block compatible with 384-well V-bottom plates for use on the FLEXMAP 3D system |
| Bar Code Scanner | CN-PC03-01 | Call for price | Handheld USB Bar Code Scanner for use with the FLEXMAP 3D instrument |
| FLEXMAP 3D Swivel Base | CN-0216-01 | Call for price | Optional swivel base that allows the user to rotate the instrument for ease of maintenance. Swivel base is commonly used when integrating to front-end liquid handlers. |
| Accessories | |||
| Syringe, 500 mL with Seal | CN-0013-01 | Call for price | Replacement syringe assembly for the FLEXMAP3D system |
| Syringe seal, (qty 4) | CN-0014-01 | Call for price | Replacement seals for FLEXMAP 3D 500 mL syringe |
| 6-Month High Throughput PM Kit | CN-0215-01 | Call for price | Preventive maintenance kit for high throughput applications. Includes HEPA air filter and 500 mL syringe seal |
| Fuse (6 AMP) | CN-0226-01 | Call for price | Replacement fuse located inside the power module. |
| Cable USB (A to B) | CN-0227-01 | Call for price | Communications cable between FLEXMAP 3D instrument and PC. |
| Air Filter HEPA | CN-0228-01 | Call for price | Replacement high efficiency air filter located behind the panel on the left side of the fluidics bay. |
| Air Filter, XY Platform | CN-0229-01 | Call for price | Replacement air filter located on the front panel of the XY module |
| Air Filter: Access Door | CN-0222-01 | Call for price | Replacement front door ventilation filters |
Downloads
| Document | Language | Type | Download |
|---|---|---|---|
| Manuals and MSDS |
|||
| FLEXMAP 3D Hardware User Manual - RUO | English | pdf  |
 |
| xPONENT 4.2 for FLEXMAP 3D Software User Manual - RUO | English | pdf |
|
| xPONENT 4.2 for FLEXMAP 3D Software Quick Guide - RUO | English | pdf |
|
| MSDS | |||
| Data Sheets and Brochures | |||
| FLEXMAP 3D Brochure | English | pdf |
|
| Luminex Instrument Comparison | English | pdf |
|
| xMAP Technology Analyte Menu | English | pdf |
|
| xMAP Technical Sheet | English | pdf |
|
FLEXMAP 3D Training Courses
| Course Title | Format | Duration |
|---|---|---|
| FLEXMAP 3D & xPONENT® 4.0 | Online | 1 hour |
| FLEXMAP 3D & xPONENT 4.0 Basics | Classroom | 1/2 day |
| FLEXMAP 3D & xPONENT 4.2 Basics | Classroom | 1/2 day |
Support Information
Luminex offers comprehensive technical support enabling companies and laboratories to perform bioassays quickly, accurately, and cost effectively while optimizing the customer experience with Luminex systems.
How To Videos
| General Routines | |
|---|---|
| Running the System Initialization Routine in the FLEXMAP 3D xPONENT Software Version 4.2 | 7:09 minutes |
| Creating a Protocol: Standards & Controls Using xPONENT 4.2 for FLEXMAP 3D | 4:06 minutes |
| Creating a Batch from an Existing Protocol Using xPONENT 4.2 for FLEXMAP 3D | 4:14 minutes |
| Using the Probe Height Adjustment Tool for 384 Well Plates on the FLEXMAP 3D | 5:25 minutes |
| Shutdown the Analyzer in xPONENT for FLEXMAP 3D Software Version 4.2 | 3:34 minutes |
| Maintenance | |
| Following the Maintenance Schedule for FLEXMAP 3D | 3:58 minutes |
| Clean the Sample Probe | 4:10 minutes |
| Replacing the Teflon Syringe Seal on the FLEXMAP 3D | 6:02 minutes |
| Replacing the HEPA Filter on the FLEXMAP 3D | 4:01 minutes |
| Removing and Replacing the Ventilation Filters on the FLEXMAP 3D | 5:00 minutes |
| Replacing the Sheath Fluid Filter on the FLEXMAP 3D | 4:05 minutes |
| Replacing the Fuses on the FLEXMAP 3D | 4:09 minutes |
| Troubleshooting | |
| Run a Clog Removal Routine with xPONENT for FLEXMAP 3D Software Version 4.2 | 2:58 minutes |
| Support Resources | Technical Support | Field Service | ||
|---|---|---|---|---|
| Protocols | Online Support Login | Certified Service Providers | ||
| Technical Tips | Email: [email protected] | Maintenance and Support | ||
| Target Values | Phone: 512-381-4397 | Luminex Central Repair Facility | ||
| Recommended Materials | Toll Free: 1-877-785-2323 | |||
| International: + 800-2939-4959 |
PC Warranty Transfer Information
Dell provides a limited warranty with PCs shipped from Luminex. Upon receipt of the Dell PC that is associated with your Luminex System, please complete the warranty transfer process. This will ensure the newly acquired PC is in your name and that you will receive technical support from Dell for the remainder of your warranty period. Required Items: Service Tag Number and Express Service Code (ESC) – located directly on the Dell PC.
